响应面法优化青春双歧杆菌增殖培养基  

Optimization of Enrichment Medium for Bifidobacterium adolescentis by Response Surface Methodology

作　　者：朱蒙蒙 李国莹[1,2] 顾秋亚[1,2] 余晓斌[1,2] ZHU Mengmeng;LI Guoying;GU Qiuya;YU Xiaobin(School of Biotechnology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122 ,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2019年第4期111-117,共7页Journal of Food Science and Biotechnology

基　　金：工业生物技术教育部重点实验室项目(KLIB-KF201105);中央高校基本科研业务费专项资金项目(JUSRP111A24);国家轻工技术与工程一流学科自主课题资助项目(LITE2018-11)

摘　　要：为提高青春双歧杆菌在发酵液中的活菌数,以MRS为培养基,采用响应面法对培养基进行优化,同时比较了优化前后的生长曲线与pH的变化。通过响应面分析结果得到青春双歧杆菌的增殖培养基配方:葡萄糖15.85g/L、低聚果糖15.85 g/L、胰蛋白胨12.04 g/L、牛肉膏7.23 g/L、酵母粉9.63 g/L、柠檬酸铵2.75 g/L、K2HPO4·3H2O 2.75 g/L、乙酸钠6.875 g/L、吐温-80 1.0 mL、MgSO4·7H2O 0.2 g/L、MnSO4·H2O 0.05 g/L、L-半胱氨酸盐酸盐0.5 g/L。该菌在增殖培养基中28 h达到稳定期,比优化前缩短12 h;活菌数达到8.9×10^9CFU/mL,是优化前的1.73倍。Response surface methodology was used to optimize the fermentation medium based on the medium MRS to increase the viable bacteria count of B.adolescentis.Meanwhile,the growth curve and pH were compared before and after optimization.According to the results of response surface analysis,the enrichment medium components were determined as follows:glucose 15.85g/L,fructo-oligosaccharide 15.85 g/L,peptone 14.45 g/L,beef extract 8.67 g/L,yeast extract power5.78 g/L,ammonium citrate 2.75 g/L,K2HPO4·3H2O 2.75 g/L,sodium acetate 6.875 g/L,Tween-801.0 mL,Mg SO4·7H2O 0.2 g/L,MnSO4·H2O 0.05 g/L,L-cystein-HCl 0.5 g/L.Cultivated in optimized enrichment medium,B.adolescentis reached stationary phase at about 28 h,which was shorter 12 h than before;the viable counts reached up to 8.9×10^9CFU/mL,which was 1.73 times as many as before.

关 键 词：青春双歧杆菌 碳氮比 正交试验 响应面法 优化 生长曲线 

分 类 号：Q815[生物学—生物工程]