文蛤酶解产物的抗氧化活性评价及其组成分析  

Antioxidant evaluation and composition characterization of enzymatic hydrolysates of Meretrix meretrix L.

作　　者：胡大伟 李恒 蒋敏 丁海龙 王松涛 沈才洪 许正宏[3,4] 史劲松 HU Dawei;LI Heng;JIANG Min;DING Hailong;WANG Songtao;SHEN Caihong;XU Zhenghong;SHI Jinsong(School of Pharmaceutical Science,Jiangnan University,Wuxi 214122,China;National Engineering Research Center for Solid-State Brewing,Luzhou 646000,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China;National Engineering Laboratory for Cereal Fermentation Technology (Jiangnan University),Wuxi 214122,China)

机构地区：[1]江南大学药学院,江苏无锡214122 [2]国家固态酿造工程技术研究中心,四川泸州646000 [3]江南大学生物工程学院,江苏无锡214122 [4]粮食发酵工艺与技术国家工程实验室(江南大学),江苏无锡214122

出　　处：《食品与发酵工业》2019年第12期207-214,共8页Food and Fermentation Industries

基　　金：国家海洋公益性行业科研专项经费项目(201305007);四川省重点研发计划项目(2017JZ0043)

摘　　要：比较不同分子质量分布的文蛤酶解产物的抗氧化活性,分析小分子酶解组分(enzymatic hydrolysate components with low molecular weight,LMEC)的组成。通过蛋白酶种类的筛选确定文蛤软体酶解工艺,以总还原力与多种自由基清除能力表征各酶解组分的抗氧化活性,采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)与高效液相色谱(high-performance liquid chromatography,HPLC)分析LMEC分子质量,采用超高压液相色谱-质谱联用技术(ultra-performance liquid chromatography and mass spectrometry,UPLC-MS)分析LMEC的肽段组成。建立了以风味蛋白酶与中性蛋白酶双酶酶解获取文蛤软体酶解物的稳定工艺流程,LMEC具有较好的抗氧化活性,其总还原力为54.16%,羟自由基、DPPH自由基及超氧阴离子清除率最大分别为53.5%、49%、47.7%。LMEC含有79%蛋白肽类,平均分子质量1.05 kDa,富含Ala、Thr、Val、Leu、Pro、Glu等氨基酸,推测了6种可能的肽段组成。该研究为文蛤抗氧化活性提供理论依据,也为后续活性肽的分离提供参考方向。Antioxidant activities of enzymatic hydrolysates of Meretrix meretrix L.with different molecular weights were compared,and the enzymatic hydrolysate components with low molecular weight(LMEC)were analyzed.A stable double-enzyme hydrolysis technique with flavor protease and neutral protease to prepare Meretrix meretrix L.hydrolysates was established.The LMEC presented relatively good antioxidant activities,as their total reducing power was 54.16%,and its maximum scavenging rates of hydroxyl radicals,DPPH free radicals and superoxide anions were 53.5%,49%,and 47.7%,respectively.Besides,LMEC included 79%oligo-peptides with an average molecular weight of 1.05 kDa,and it was rich in Ala,Thr,Val,Leu,Pro,and Glu.According to UPLC-MS analysis,LMEC were speculated to have six possible peptides.This study provides a theoretical basis for understanding the antioxidant activity of Meretrix meretrix L.and also for separating active peptides in the future.

关 键 词：文蛤 酶解 抗氧化 组成分析 肽 

分 类 号：O657.63[理学—分析化学] TS254.1[理学—化学]