HPLC-ELSD法检测无糖型饮料中D-阿洛酮糖  

Determination of D-psicose in Sugar-free Beverage by HPLC-ELSD

作　　者：刘文全[1] LIU Wen-quan(Panzhihua University, Panzhihua 617000, Sichuan, China)

机构地区：[1]攀枝花学院

出　　处：《食品研究与开发》2019年第14期197-202,共6页Food Research and Development

摘　　要：建立一种用高效液相色谱-蒸发光检测法测定无糖型饮料中D-阿洛酮糖的方法.样品经20%乙腈提取后,由HLB固相萃取小柱进行净化和富集,过滤膜后上机检测.检测结果表明,以乙腈:水(30:70,体积比)作为流动相,用蒸发光检测器检测,外标法峰面积定量,D-阿洛酮糖在0.01 g/100 mL^0.80 g/100 mL浓度范围内线性良好,相关系数r2为0.9994,检出限在0.002 g/100 mL,定量限为0.006 g/100 mL,加标回收率达到91.5%~94.2%.该方法适用于无糖型饮料中D-阿洛酮糖的定量检测.A method for the determination of D-psicose sugar in sugar-free beverages by high performance liquid chromatography-evaporative light detection was established. After 20 % acetonitrile extraction, the samples were purified and enriched by HLB solid phase extraction column, and then detected on the machine after filtration membrane.The separation of targeted compound used acetonitrile ∶ water (30 ∶ 70,volume ratio) as mobile phase, with evaporative light scattering detector and external standard method peak area quantification. The linear range of D-psicose was in the range of 0.01 g/100 mL-0.80 g/100 mL with a correlation coefficient of 0.999 4. The detection limit was 0.002 g/100 mL and the quantitative limit was 0.006 g/100 mL. The recovery rate was 91.5 %-94.2 %. The method is suitable for the quantitative detection of D-psicose in sugar-free beverage.

关 键 词：高效液相色谱 蒸发光散射检测器 无糖型 饮料 D-阿洛酮糖 

分 类 号：O657[理学—分析化学] TS27[理学—化学]