电针干预对大鼠脑缺血再灌注损伤的神经保护作用及其机制  

Neuroprotective effect of electro-acupuncture on rats with cerebral ischemia-reperfusion injury and its mechanism

作　　者：张吉芳 韩冰[3] 孙国栋[2] ZHANG Jifang;HAN Bing;SUN Guodong(Jinan University,Jinan 250000,China)

机构地区：[1]济南大学·山东省医学科学院医学与生命科学学院,济南250000 [2]山东省医学科学院附属医院·山东第一医科大学 [3]济宁市疾病预防控制中心

出　　处：《山东医药》2019年第19期35-38,共4页Shandong Medical Journal

摘　　要：目的探讨电针干预对大鼠脑缺血再灌注损伤的神经保护作用及其机制。方法选择成年雄性SD大鼠90只,随机分为假手术组、模型组和电针组,每组30只。模型组和电针组通过改良线栓法建立局灶性脑缺血再灌注损伤模型;假手术组仅分离血管,不进行阻闭;电针组造模结束即刻电针刺激百会穴、大椎穴,每12h治疗1次,每次30min,共治疗5次。各组分别于再灌注6、24、48h,采用Longa法进行神经功能缺失评分;各组再灌注48h,采用激光多普勒血流仪检测缺血侧局部脑血流量和血流速度;各组随机选取10只,断头处死,迅速取出大脑组织,均匀切片,TTC染色,计算脑梗死体积百分比;各组随机选取10只,断头处死,取缺血侧脑皮质,采用TUNEL染色法检测神经细胞凋亡率;剩余大鼠,断头处死,取脑组织,采用Western blotting法检测Wnt/β-catenin信号通路关键因子β-catenin蛋白及凋亡相关因子Bcl-2、Bax蛋白表达。结果与假手术组比较,模型组脑血流量和血流速度明显降低,神经功能缺失评分、脑梗死面积百分比以及神经细胞凋亡率均明显升高,脑组织Bax蛋白表达明显升高,而β-catenin、Bcl-2蛋白表达明显降低(P均<0.05)。与模型组比较,电针组脑血流量和血流速度均明显升高,神经功能缺失评分、脑梗死面积百分比以及神经细胞凋亡率均明显降低,脑组织Bax蛋白表达明显降低,而β-catenin、Bcl-2蛋白表达明显升高(P均<0.05)。结论电针干预对大鼠脑缺血再灌注损伤具有神经保护作用,其作用机制与激活Wnt/β-catenin信号通路及抑制Bax蛋白表达、促进Bcl-2蛋白表达有关。ObjectiveTo investigate the neuroprotective effect of electro-acupuncture on cerebral ischemia-reperfusion injury rats and its mechanism. Methods Ninety adult male SD rats were randomly divided into the sham operation group, model group and electro-acupuncture group, with 30 rats in each. The focal cerebral ischemia-reperfusion injury rat models in the model group and electro-acupuncture group were established by modified thread embolism method;the rats in the shamoperation group were only given blood vessel isolation without blocking blood vessel;the rats in the electro-acupuncture group were given electro-acupuncture at Baihui and Dazhui acupoint immediately after modeling, once every 12 h, 30 min each time, for 5 times. At 6, 24 and 48 h after reperfusion, Longa method was used to score the neurological deficit. At 48 h after reperfusion, laser Doppler flow meter was used to detect the regional cerebral blood flow and velocity in the ischemic side. Ten rats in each group were randomly selected and decapitated to death, and their brain tissues were taken out quickly, evenly sliced. TTC staining was used to calculate the percentage of cerebral infarction volume;ten rats in each group were randomly selected and decapitated to death, and the cerebral cortex of ischemic side was taken to detect the apoptotic rate of neurons by TUNEL staining. The remaining rats were decapitated and their brain tissues were taken. The expression of Wnt/β-catenin signaling pathway key factor β-catenin protein, apoptosis-related factors Bcl-2 and Bax protein in brain tissues were detected by Western blotting. Results Compared with the sham operation group, the cerebral blood flow and velocity decreased significantly, the scores of neurological deficit, the percentage of cerebral infarction area and the apoptotic rate of neurons increased, the expression of Bax protein in the brain tissue increased, and the expression of β-catenin and Bcl-2 protein decreased significantly in the model group (all P<0.05). Compared with the model grou

关 键 词：脑缺血再灌注损伤 电针疗法 细胞凋亡 WNT/Β-CATENIN信号通路 

分 类 号：R743[医药卫生—神经病学与精神病学]