Sporamin suppresses growth of xenografted colorectal carcinoma in athymic BALB/c mice by inhibiting liver β-catenin and vascular endothelial growth factor expression  

作　　者：Chun Yang Jing-Jie Zhang Xiao-Peng Zhang Rong Xiao Peng-Gao Li 

机构地区：[1]School of Public Health,Capital Medical University,Beijing Key Laboratory of Clinical Epidemiology

出　　处：《World Journal of Gastroenterology》2019年第25期3196-3206,共11页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.81573128 and No.81703216

摘　　要：BACKGROUND Colorectal cancer(CRC)is the third most common malignancy of the digestive tract and the fifth leading cause of cancer-related mortality in China.Sporamin,a Kunitz-type trypsin inhibitor isolated from sweet potato,is a potential anti-cancer agent with activities against a number of malignant tumor cells in vitro.The liver secretes a myriad of endocrine factors that may facilitate the growth and transformation of tumors in the development of CRC.AIM To investigate the effects of sporamin on liver morphology and biomarkers of xenografted CRC in the liver of athymic BALB/c mice.METHODS Twenty-seven male BALB/c nude mice were randomly divided into control,vehicle,and sporamin groups.Mice in the latter two groups were intraperitoneally xenografted with LoVo colorectal carcinoma cells and intragastrically infused with saline or sporamin(0.5 g/kg body weight/d),respectively,for 3 wk.Hematoxylin and eosin(HE)staining of the sections was performed to observe morphological changes in hepatic tissue and real-time fluorescent quantitative PCR(qPCR)and enzyme-linked immunosorbent assay(ELISA)were used to measure the expression ofβ-catenin and vascular endothelial growth factor(VEGF)in the liver.RESULTS Sporamin significantly reduced the number and weight of tumor nodules formed in the abdominal cavity.Compared with the vehicle group,the mean tumor weight(±SD)in the sporamin group was significantly reduced(0.44±0.10 g vs 0.26±0.15 g)and the total number of tumors decreased from 93 to 55.HE staining showed that enlargement of the nucleus and synthesis of proteins within hepatocytes,as well as infiltration of inflammatory cells into the liver,were attenuated by sporamin.Immunohistochemical staining and ELISA showed that the concentrations ofβ-catenin and VEGF in the liver were significantly reduced by sporamin.Compared with the vehicle group,the expression ofβ-catenin measured in integrated optical density units per area was reduced in the sporamin group(47.29±9.10 vs 26.14±1.72;P=0.003).Expression of VEGF wasBACKGROUND Colorectal cancer(CRC) is the third most common malignancy of the digestive tract and the fifth leading cause of cancer-related mortality in China. Sporamin, a Kunitz-type trypsin inhibitor isolated from sweet potato, is a potential anti-cancer agent with activities against a number of malignant tumor cells in vitro. The liver secretes a myriad of endocrine factors that may facilitate the growth and transformation of tumors in the development of CRC.AIM To investigate the effects of sporamin on liver morphology and biomarkers of xenografted CRC in the liver of athymic BALB/c mice.METHODS Twenty-seven male BALB/c nude mice were randomly divided into control,vehicle, and sporamin groups. Mice in the latter two groups were intraperitoneally xenografted with LoVo colorectal carcinoma cells and intragastrically infused with saline or sporamin(0.5 g/kg body weight/d),respectively, for 3 wk. Hematoxylin and eosin(HE) staining of the sections was performed to observe morphological changes in hepatic tissue and real-time fluorescent quantitative PCR(qPCR) and enzyme-linked immunosorbent assay(ELISA) were used to measure the expression of β-catenin and vascular endothelial growth factor(VEGF) in the liver.RESULTS Sporamin significantly reduced the number and weight of tumor nodules formed in the abdominal cavity. Compared with the vehicle group, the mean tumor weight(± SD) in the sporamin group was significantly reduced(0.44 ± 0.10 g vs0.26 ± 0.15 g) and the total number of tumors decreased from 93 to 55. HE staining showed that enlargement of the nucleus and synthesis of proteins within hepatocytes, as well as infiltration of inflammatory cells into the liver, were attenuated by sporamin. Immunohistochemical staining and ELISA showed that the concentrations of β-catenin and VEGF in the liver were significantly reduced by sporamin. Compared with the vehicle group, the expression of β-catenin measured in integrated optical density units per area was reduced in the sporamin group(47.29 ± 9.10 vs 26.14 ± 1.

关 键 词：SPORAMIN Colorectal cancer LIVER Vascular ENDOTHELIAL GROWTH factor Β-CATENIN 

分 类 号：R735.3[医药卫生—肿瘤]