Nuclear magnetic resonance-based metabolomics and metabolic pathway networks from patient-matched esophageal carcinoma,adjacent noncancerous tissues and urine  

作　　者：Jia-Hao Liang Yan Lin Ting Ouyang Wan Tang Yao Huang Wei Ye Jia-Yun Zhao Zhe-Ning Wang Chang-Chun Ma 

机构地区：[1]Department of Radiology,Second Affiliated Hospital,Shantou University Medical College,Shantou 515041,Guangdong Province,China [2]Department of Radiation Oncology,Affiliated Tumor Hospital,Shantou University Medical College,Shantou 515041,Guangdong Province,China

出　　处：《World Journal of Gastroenterology》2019年第25期3218-3230,共13页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.81471729 and No.81101102;the Science and Technology and Planning Project of Guangdong Province,No.2016A020216025;the Research Award Fund for Outstanding Young Teachers in Higher Education Institutions,Guangdong Province,No.YQ2015245;the National Natural Science Foundation of Guangdong Province,No.S2011010004973;the Department of Education of Guangdong Province,No.2017KTSCX071

摘　　要：BACKGROUND Several studies have demonstrated a correlation between esophageal cancer(EC)and perturbed urinary metabolomic profiles,but none has described the correlation between urine metabolite profiles and those of the tumor and adjacent esophageal mucosa in the same patient.AIM To investigate how urinary metabolic phenotypes were linked to the changes in the biochemical landscape of esophageal tumors.METHODS Nuclear magnetic resonance-based metabolomics were applied to esophageal tumor tissues and adjacent normal mucosal tissues alongside patient-matched urine samples.RESULTS Analysis revealed that specific metabolite changes overlapped across both metrics,including glucose,glutamate,citrate,glycine,creatinine and taurine,indicating that the networks for metabolic pathway perturbations in EC,potentially involved in but not limited to disruption of fatty acid metabolism,glucose and glycolytic metabolism,tricarboxylic acid cycle and glutaminolysis.Additionally,changes in most urinary biomarkers correlated with changes in biomarker candidates in EC tissues,implying enhanced energy production for rapid cell proliferation.CONCLUSION Overall,these associations provide evidence for distinct metabolic signatures and pathway disturbances between the tumor tissues and urine of EC patients,and changes in urinary metabolic signature could reflect reprogramming of the aforementioned metabolic pathways in EC tissues.Further investigation is needed to validate these initial findings using larger samples and to establish the underlying mechanism of EC progression.BACKGROUND Several studies have demonstrated a correlation between esophageal cancer(EC)and perturbed urinary metabolomic profiles, but none has described the correlation between urine metabolite profiles and those of the tumor and adjacent esophageal mucosa in the same patient.AIM To investigate how urinary metabolic phenotypes were linked to the changes in the biochemical landscape of esophageal tumors.METHODS Nuclear magnetic resonance-based metabolomics were applied to esophageal tumor tissues and adjacent normal mucosal tissues alongside patient-matched urine samples.RESULTS Analysis revealed that specific metabolite changes overlapped across both metrics, including glucose, glutamate, citrate, glycine, creatinine and taurine,indicating that the networks for metabolic pathway perturbations in EC,potentially involved in but not limited to disruption of fatty acid metabolism,glucose and glycolytic metabolism, tricarboxylic acid cycle and glutaminolysis.Additionally, changes in most urinary biomarkers correlated with changes in biomarker candidates in EC tissues, implying enhanced energy production for rapid cell proliferation.CONCLUSION Overall, these associations provide evidence for distinct metabolic signatures and pathway disturbances between the tumor tissues and urine of EC patients, and changes in urinary metabolic signature could reflect reprogramming of the aforementioned metabolic pathways in EC tissues. Further investigation is needed to validate these initial findings using larger samples and to establish the underlying mechanism of EC progression.

关 键 词：Esophageal cancer Metabolites Metabolic pathways Nuclear magnetic resonance-based metabolomics Tumor tissue URINE 

分 类 号：R[医药卫生]