下调miRNA-214表达抑制胃癌SGC-7901/DDP细胞顺铂耐药、迁移和上皮间质转化  

Downregulation of miRNA-214 inhibits cisplatin resistance, cell migration, and epithelial interstitial transformation in gastric cancer SGC-7901/DDP cells

作　　者：朱艳[1] 刘玮丽 吴明东[1] 庄永卫 叶淑芳[1] 施旭红[1] Yan Zhu;Yu-Li Liu;Ming-Dong Wu;Yong-Wei Zhuang;Shu-Fang Ye;Xu-Hong Shi(Department of General Practice, Lishui People’s Hospital,Lishui 323000, Zhejiang Province, China;Department of Gastroenterology, Run Run Shaw Hospital,Hangzhou 310000, Zhejiang Province, China)

机构地区：[1]丽水市人民医院全科,浙江省丽水市323000 [2]邵逸夫医院消化内科,浙江省杭州市310000

出　　处：《世界华人消化杂志》2019年第12期742-747,共6页World Chinese Journal of Digestology

基　　金：丽水市科技计划项目,No.2015zdxk07~~

摘　　要：背景胃癌(gastric cancer, GC)是常见的消化系统肿瘤之一,而肿瘤化疗耐药是制约GC癌治疗效果的瓶颈,探索逆转GC化疗耐药机制对于GC的治疗具有重大意义.目的探讨敲低miR-214表达对GC细胞SGC-7901/DDP顺铂耐药性、迁移以及上皮间质转化(epithelial mesenchymaltransformation, EMT)的影响,并讨论初步作用机制.方法采用LipofectamineTM2000法转染miR-214 inhibitor至SGC-7901/DDP细胞后, CCK-8法评估细胞对顺铂耐药性的改变;划痕试验检测细胞迁移能力;免疫印迹法检测细胞中E-钙黏蛋白(E-cad)、波形蛋白(Vimentin)和N-钙黏蛋白(N-cad),以及NF-κB与Bcl-2蛋白表达水平.结果转染miR-214 inhibitor后, SGC-7901/DDP细胞对顺铂耐药性明显降低(P <0.05);且细胞迁移能力明显降低(P <0.05), E-cad蛋白表达显著升高(P <0.05), Vimentin与N-c a d蛋白表达均显著降低(均P <0.05).另外,SGC-7901/DDP细胞中NF-κB与Bcl-2的表达明显降低(均P <0.05).结论下调miR-214表达具有降低GCSGC-7901/DDP细胞对顺铂耐药性,抑制细胞迁移与EMT的作用.而这种作用可能与下调NF-κB与Bcl-2的表达有关.BACKGROUND Gastric cancer (GC) is one of the common tumors of the digestive system, and resistance to chemotherapy is the bottleneck that restricts the therapeutic effect of chemotherapy in GC. Therefore, it is of great significance to explore the mechanism of reversal of chemotherapy resistance. AIM To investigate the effect of miRNA-214 (miR-214) knockdown on cisplatin resistance, cell migration, and epithelial mesenchymal transformation (EMT) in GC SGC-7901/DDP cells, and to explore the preliminary mechanism. METHODS After transfection of SGC-7901/DDP cells with miR-214 inhibitor using the LipofectamineTM2000 method, the change of cell resistance to cisplatin was evaluated by the CCK-8 assay, cell migration was evaluated by wound healing assay, and the protein expression levels of E-cadherin (E-cad), Vimentin, N-cadherin (N-cad), NF-κB, and Bcl-2 were detected by Western blot. RESULTS After transfection with miR-214 inhibitor, the resistance of SGC-7901/DDP cells to cisplatin was significantly reduced (P < 0.05), and the ability of cell migration was obviously decreased (P < 0.05). The expression level of E-cad protein was significantly increased (P < 0.05), while the expression levels of Vimentin and N-cad proteins were significantly decreased (P < 0.05). Moreover, the expression levels of NF-κB and Bcl-2 protein in SGC-7901/DDP cells were significantly decreased (P < 0.05). CONCLUSION Down-regulation of miR-214 can reduce the resistance of SGC-7901/DDP cells to cisplatin and inhibit cell migration and EMT. This effect may be related to the down-regulation of NF-κB and Bcl-2 expression.

关 键 词：miR-214 胃癌 上皮间质转化 顺铂耐药 

分 类 号：R735.2[医药卫生—肿瘤]