骨形成蛋白2、磷酸酶基因及张力蛋白同源物在初发、复发及恶变涎腺多形性腺瘤组织中的表达及意义  

Expression and significance of Bone Morphogenetic Protein 2 and phosphatase and tensin homolog deleted on chromosome ten in incipient,recurrent and malignant salivary gland pleomorphic adenoma

作　　者：李冠娥[1] 范新昊 李海洋[1] 代振杰 李金源 Li Guan-e;Fan Xinhao;Li Haiyang;Dai Zhenjie;Li Jinyuan(Department of Head and Neck surgery,Kailuan General Hospital,Tangshan 063000,China;Stomatology college of North China University of Science and Technology,Tangshan 063000,China)

机构地区：[1]开滦总医院头颈外科,河北省唐山市063000 [2]华北理工大学口腔医学院,河北省唐山市063000

出　　处：《中国综合临床》2019年第4期305-310,共6页Clinical Medicine of China

基　　金：河北省医学科学研究重点课题(20150849);河北省科技厅重点研发计划项目(182777101d).

摘　　要：目的探讨骨形成蛋白2(bone morphogenetic protein-2,BMP-2)及磷酸酶基因及张力蛋白同源物(phosphatase and tensin homolog deleted on chromosome ten,PTEN)在初发、复发及恶变的涎腺多形性腺瘤(pleomorphic adenoma,PA)组织中的表达和意义。方法收集开滦总医院2008年至2017年PA 93例,其中初发组54例(A组,良性47例为A1组,恶性7例为A2组),复发组39例(B组,良性26例为B1组,恶性13例为B2组),通过免疫组化及Western检测BMP-2、PTEN在各组标本中的表达情况,Spearman相关分析探讨BMP-2、PTEN表达水平与涎腺多形性腺瘤进展的相关性。结果免疫组化与Western blot检测结果一致:BMP-2在PA复发组表达显著高于初发组(129.03±15.52与87.88±18.11,t=-8.094,P=0.000),在恶性病例表达显著低于良性病例(100.24±25.07与116.66±26.19,t=2.125,P=0.040);PTEN在复发组的表达与初发组差异无统计学意义(89.15±13.92与96.19±28.02,t=1.055,P=0.279),在恶性病例表达显著低于良性病例(76.06±11.16与109.28±17.05,t=7.543,P=0.000);BMP-2与PTEN的表达存在正相关(r=0.313;P<0.05)。结论BMP-2可能与涎腺多形性腺瘤的复发有关;BMP-2和PTEN可能均与涎腺多形性腺瘤的恶变有关。Objective To investigate the expression and significance of bone morphogenetic protein-2(BMP-2)and phosphatase and tensin homolog deleted on chromosome ten(PTEN)in incipient,recurrent and malignant salivary gland pleomorphic adenoma.Methods A total of 93 cases of salivary gland pleomorphic adenoma in Kailuan general hospital from 2008 to 2017 were collected,including 54 in incipient group(group A,47 cases in benign group A1,7 cases in malignant group A2),39 cases in recurrent group(group B,26 cases in benign group B1,13 cases in malignant group B2).The expression of BMP-2 and PTEN were detected by immunohistochemical detection and western blot,the correlation of BMP-2 and PTEN was analyzed by Spearman analysis.Results The immunohistochemical and western blot analysis both showed expression of BMP-2 in recurrent group was significantly higher than that in incipient cases((129.03±15.52)vs.(87.88±18.11),t=-8.094,P=0.000),and it was lower in malignant cases than that in benign cases((100.24±25.07)vs(116.66±26.19),t=2.125,P=0.040).There was no significant difference in PTEN expression between incipient and recurrent groups((89.15±13.92)vs.(96.19±28.02),t=1.055,P=0.279),but lower PTEN expression was found in malignant cases than benign cases((76.06±11.16)vs.(109.28±17.05),t=7.543,P=0.000).BMP-2 was positively correlated with PTEN expression(r=0.313,P<0.05).Conclusion BMP-2 is associated with the recurrence of salivary gland pleomorphic adenoma,and both BMP-2 and PTEN are associated with malignant in the salivary gland pleomorphic adenoma.

关 键 词：多形性腺瘤 骨形成蛋白2 磷酸酶基因及张力蛋白同源物 免疫组化 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]