AtDREB1A基因过量表达对马铃薯生长及抗非生物胁迫基因表达的影响  

Effects of over-expression of AtDREB1A gene on potato growth and abiotic stress resistance gene expression

作　　者：贾小霞[1,2] 齐恩芳 刘石[1,2] 文国宏 马胜[1,2] 李建武 黄伟 JIA Xiao-Xia;QI En-Fang;LIU Shi;WEN Guo-Hong;MA Sheng;LI Jian-Wu;HUANG Wei(Potato Research Institute,Gansu Academy of Agricultural Sciences/Gansu Engineering Laboratory of Potato Germplasm Resources Innovation,Lanzhou 730070,Gansu,China;The Ministry of Agriculture,Scientific Observation and Experiment Station of Dry Potato in the Northwest,Weiyuan 748201,Gansu,China)

机构地区：[1]甘肃省农业科学院马铃薯研究所/甘肃省马铃薯种质资源创新工程实验室,甘肃兰州730070 [2]农业部西北旱作马铃薯科学观测实验站,甘肃渭源748201

出　　处：《作物学报》2019年第8期1166-1175,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31560412,31060200);甘肃省农业科学院科技创新专项(2017GAAS38);国家现代农业产业技术体系建设专项(CARS-09-P06)资助~~

摘　　要：为明确AtDREB1A基因过量表达对马铃薯生长及基因表达的影响,以马铃薯品种陇薯3号(L3)及其AtDREB1A转基因株系T2为材料,采用盆栽试验,在马铃薯盛花期将盆土含水量控制为田间最大持水量(FWC)的45%~50%,观察转基因前后植株表型,并研究叶片MDA含量、RWC、SOD和POD活性及其基因表达的差异。结果表明,正常浇水条件下2个株系各指标差异不大。胁迫20d后,转基因植株T2的表型明显好于对照L3,且RWC显著高于L3;各株系叶片的MDA含量、SOD和POD活性均明显上升,但转基因植株MDA含量上升幅度较对照小,抗氧化保护酶SOD、POD活性升高幅度较对照大,说明转基因植株细胞膜损伤和膜脂过氧化程度较轻,植株的耐旱性明显提高。转录组测序及生物信息学分析发现,转基因材料T2相对于L3的差异表达基因共430个,其中上调表达基因287个,下调表达基因143个。功能注释和显著性富集结果表明,差异表达基因富集涉及GO功能分类体系中生物过程、细胞组分和分子功能3个大类别,且大部分集中在细胞内和膜上,主要涉及信号传导、氧化还原、生物调解、应激反应、发育过程、系统免疫过程、核酸和蛋白结合转录因子活性、转运活性及催化活性。其中抗非生物胁迫相关蛋白PPR、HSP、P450、MLO等家族的大量基因表达量发生较大变化,说明这些基因在转AtDREB1A基因马铃薯抵御干旱过程中发挥着非常重要的作用。本研究为进一步解析AtDREB1A基因提高马铃薯抗旱性的调控网络奠定了基础。Longshu 3 (L3) and its AtDREB1A transgenic line T2 were planted in the pot experiment. The water content of the soil in pots was controlled to 45%–50% of the maximum water holding capacity (FWC) during the flowering stage of the potato. The phenotype, MDA content, RWC, activities of SOD and POD, and the gene expression level in leaves were analyzed, showing there were no significant differences between the two lines under normal watering conditions. After 20 d of stress treatment, the phenotype of transgenic plants T2 was significantly better than that of control L3, and RWC was significantly higher than that of L3. The MDA content, SOD and POD activities of leaves in each line increased significantly, while the MDA content of transgenic plants increased less than, and the activities of SOD and POD of transgenic plants increased more than those of the control, indicating that the cell membrane damage and membrane lipid peroxidation of the transgenic plants were lighter than those of the control, and the drought tolerance of transgenic plants was significantly improved. Transcriptome sequencing and bioinformatic analysis showed that compared with L3, there were 430 differentially expressed genes in T2, including 287 up-regulated genes and 143 down-regulated genes. Functional annotation and significance enrichment showed that all differentially expressed genes were involved in three broad categories of GO functional classification systems, that was biological processes, cell components and molecular functions. Most of these differentially expressed genes concentrated on the membrane in cells, and mainly related to signal transduction, redox, biological mediation, stress response, development process, system immune process, nucleic acid and protein binding transcription factor activity, transport activity and catalytic activity. The expression of a large number of abiotic stress-related genes was up-regulated, including PPR protein family, P450 protein family, heat shock protein family and MLO protein family, indi

关 键 词：马铃薯 AtDREB1A基因 非生物胁迫 差异表达基因 干旱 

分 类 号：S532[农业科学—作物学]