苦参素对大鼠全脑缺血再灌注损伤保护作用的实验研究  

Protective Effects of Oxymatrine on Global Cerebral Ischemia-reperfusion Injury in Rats

作　　者：余婷[1] 韩亚非[2] YU Ting;HAN Yafei(Handan Hospital of Traditional Chinese Medicine,Handan 056001,Hebei,China)

机构地区：[1]邯郸市中医院,河北邯郸056001 [2]邯郸市中心医院

出　　处：《中西医结合心脑血管病杂志》2019年第12期1807-1810,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：邯郸市科学技术研究与发展计划项目(No.1723208007ZC)

摘　　要：目的探讨苦参素对大鼠全脑缺血再灌注后脑组织的保护作用。方法通过夹闭血管20min复制大鼠全脑缺血再灌注模型并随机分为假手术组(0.9%氯化钠溶液)、模型组(0.9%氯化钠溶液)和苦参素低剂量组[25mg/(kg·d)]、中剂量组[50mg/(kg·d)]、高剂量组[100mg/(kg·d)],各20只。术前10min腹腔注射给药,再灌注6h后检测相关指标,记录翻正反射及脑电恢复时间,计算脑组织含水量,通过苏木精-伊红(HE)染色、末端脱氧核苷酰基转移酶介导dUTP切口末端标记(TUNEL)法分别观察海马CA1区神经元形态及凋亡状况;测定海马组织炎症细胞因子含量,生化分析脑组织抗氧化酶活性和丙二醛(MDA)含量。结果与模型组比较,苦参素中剂量组、高剂量组翻正反射和脑电恢复时间均显著缩短(P<0.01),脑组织含水量显著降低(P<0.05或P<0.01),海马CA1区神经元病理性形态结构变化和凋亡状况均明显改善,凋亡指数显著降低(P<0.01),炎症细胞因子[白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]含量均显著降低(P<0.05或P<0.01),抗氧化酶[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)]活性升高,MDA含量降低(P<0.05或P<0.01)。结论苦参素对全脑缺血再灌注损伤具有保护作用,可能与抑制氧化应激和炎症反应有关。Objective To explore the protective effect of oxymatrine on global cerebral ischemia-reperfusion in rats.Methods The rat models of global cerebral ischemia-reperfusion were replicated by clamping the four vessels for 20 minutes and were randomly assigned to sham group (0.9% sodium chloride solution),model group (0.9% sodium chloride solution)and oxymatrine low dose group[oxymatrine 25 mg/(kg·d)],medium dose group[oxymatrine 50 mg/(kg·d)],and high dose group [oxymatrine 100 mg/(kg·d)],n =20.The drugs were given by intraperitoneal injection 10 minutes before operation.Six hours after reperfusion,the time of righting reflex and EEG recovery were recorded.Content of water in brain was measured.The morphological changes of neurons in hippocampus CA1 region were observed by HE Staining.The cell apoptosis was observed after TUNEL staining,The levels of inflammatory factors,antioxidant enzymes,and malondialdehyde (MDA) in hippocampus tissue were measured.Results Compared with the model group,the time of righting reflex and EEG recovery,content of water in brain of medium dose groups and high dose groups were significantly reduced( P <0.05 or P <0.01).Hippocampal CA1 neuronal pathological changes and apoptosis were significantly improved( P < 0.05 or P <0.01).The apoptosis index (AI)was significantly decreased( P <0.01).Inflammatory factors[interleukin(IL)-1β,IL-6,tumor necrosis factor-α(TNF-α)]were significantly reduced( P <0.05 or P <0.01),antioxidant enzymes[superoxide dismutase (SOD),and catalase(CAT)] activity were significantly improved and malondialdehyde(MDA) was decreased( P <0.05 or P <0.01).Conclusion Oxymatrine has protective effects on global cerebral ischemia-reperfusion injury,which may be related to inhibition of oxidative stress and inflammatory response.

关 键 词：脑缺血再灌注损伤 大鼠 苦参素 氧化应激 细胞凋亡 炎症因子 

分 类 号：R741[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]