绿萝花化学成分及其抗氧化活性  

Chemical constituents from Edgeworthia gardneri and their antioxidant activities

作　　者：董俊丽[1] 黄传奇[1] 黄伟[1] 马浩然[1] 刘剑敏[1] DONG Jun-li;HUANG Chuan-qi;HUANG Wei;MA Hao-ran;LIU Jian-min?(Wuhan No. 1 Hospital, Wuhan 430022, China)

机构地区：[1]武汉市第一医院

出　　处：《中成药》2019年第7期1578-1582,共5页Chinese Traditional Patent Medicine

基　　金：湖北省自然科学基金青年项目(2018CFB318)

摘　　要：目的研究绿萝花Edgeworthia gardneri(Wall.) Meisn.的化学成分及其抗氧化活性。方法绿萝花乙醇提取物采用硅胶、MCI、反相ODS柱、Sephadex LH-20凝胶柱色谱等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。再采用DPPH法评价其抗氧化活性。结果从中分离得到12个化合物,分别鉴定为白瑞香素(1)、瑞香素(2)、西瑞香素(3)、伞形花内酯(4)、东莨菪内酯(5)、7-(2,3-环氧-3-甲氧基丁氧基)-香豆素(6)、异莨菪内酯(7)、5-十五烷基间苯二酚(8)、3,4-二羟基苯甲酸(9)、异绿原酸B(10)、异绿原酸C(11)、异槲皮苷(12)。所有化合物EC500.131~0.713μmol/L。结论化合物5~11为首次从该植物中分离得到。所有化合物均有良好的抗氧化活性,其中9~11与阳性对照品相当。AIM To study the chemical constituents from Edgeworthia gardneri(Wall.) Meisn. and their antioxidant activities.METHODS The ethanol extract from E. gardneri was isolated and purified by silica, MCI, ODS and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antioxidant activities were evaluated by DPPH method.RESULTS Twelve compounds were isolated and identified as edgeworthin(1),daphnetin(2),daphnoretin(3),umbelliferone(4),scopoletin(5),7-(2,3-epoxy-3-methyl-3-butyloxy)-6-methoxycoumarin(6),iscopoletin(7),5-pentadecylbenzene-1,3-diol(8),3,4-dihydroxybenzoic acid(9),isochlorogenic acid B(10),isochlorogenic acid C(11) and isoquercitrin(12).EC50 values of all the compounds were 0.131-0.713 μmol/L.CONCLUSION Compounds 5-11 are isolated from this plant for the first time.All the compounds have good antioxidant activities, and 9-11 exhibit similar antioxidant activities to positive control.

关 键 词：绿萝花 化学成分 分离鉴定 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]