槟榔碱涂擦对颊黏膜活检术后SD大鼠生存的影响  

Effect of arecoline on SD rat survival after buccal mucosal biopsy

作　　者：杨博 符梦凡 唐瞻贵 YANG Bo;FU Mengfan;TANG Zhangui(Xiangya School of Stomatology,Central South University,Changsha 410008,China)

机构地区：[1]中南大学湘雅口腔医学院

出　　处：《口腔疾病防治》2019年第7期423-427,共5页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目(81671003)

摘　　要：目的研究槟榔碱涂擦或机械刺激建立SD大鼠颊黏膜下纤维化动物模型的活检术后的生存状况,为动物实验样本量预测提供实验依据。方法将48只大鼠分为8组,每组6只。A^D组为单纯不同浓度槟榔碱(0、0.5、2、8mg/mL)涂擦颊黏膜,E^H组为不同浓度槟榔碱(0、0.5、2、8mg/mL)涂擦联合机械刺激颊黏膜。16周后活检取直径6mm颊黏膜组织块后间断缝合。记录大鼠生存时间,分析大鼠生存期与不同槟榔碱浓度和机械刺激的关系。结果所有大鼠在前16周处理至活检时均未死亡。其中,单纯2mg/mL槟榔碱刺激口腔黏膜下纤维化建模成功率为66.7%。所有SD大鼠活检术后的平均观察时间为42.5d,截止大鼠颊黏膜创伤后120d,8组的累积生存率分别(按组别字母顺序)为50%、33%、17%、0%、33%、17%、0%、0%。0mg/mL(A组和E组)、0.5mg/mL(B组和F组)、2mg/mL(C组和G组)、8mg/mL(D组和H组)的累积生存率分别为42%、25%、8%、0%。COX生存分析结果显示中高浓度槟榔碱(2、8mg/mL)显著影响大鼠生存时间(P<0.05),机械刺激对生存时间影响不显著(P>0.05)。卡方检验结果显示伤口愈合的大鼠存活率(33.3%)高于伤口未完全愈合的大鼠存活率(0.0%),差异具有统计学意义(P=0.003)。结论中高浓度槟榔碱涂擦大鼠颊黏膜的口腔黏膜下纤维化建模成功率较高,但活检术后生存时间明显下降;而机械刺激不会导致生存时间的显著下降,伤口不愈合可能为大鼠死亡的原因之一。Objective To provide an experimental basis for predicting the sample size needed for animal experiments by studying the survival of SD rats after buccal mucosal biopsy with arecoline administered at different concentrations with different methods.Methods In all,48 rats were divided into 8 groups,with 6 in each group,as follows:rats in groups A-D were treated with arecoline at different concentrations(0,0.5,2,8 mg/mL);rats in groups E-H were treated with arecoline at different concentrations(0,0.5,2,8 mg/mL),followed by stimulation of the buccal mucosa by mechanical rubbing.After 16 weeks,a 6-mm-diameter sample of the buccal mucosa was collected,and the wound was closed with interrupted sutures.The survival time of the rats was recorded,and the relationship between the survival time and the concentration of arecoline and mechanical stimulation was analyzed.Results No rats died during the first16 weeks after treatment or after biopsy.The success rate of the areoline stimulation model was 66.7%.The average observation time of all SD rats after biopsy was 42.5 days.Up to 120 days after biopsy,the cumulative survival rate in the eight groups was 50%,33%,17%,0%,33%,17%,0% and 0%,respectively(in alphabetical order).The cumulative survival rate in the groups administered 0 mg/mL(groups A and E),0.5 mg/mL(groups B and F),2 mg/mL(groups C and G),and 8 mg/mL(groups D and H)was 42%,25%,8% and 0%,respectively.Cox survival analysis showed that moderate and High concentrations of arecoline(2,8 mg/mL)significantly affected the survival duration(P<0.05),while mechanical stimulation had no significant effect on the survival duration(P>0.05).The chi-squared test showed that the survival rate of rats showing wound healing(33.3%)was significantly higher than that of rats showing incomplete wound healing(0.0%)(P=0.003).Conclusion The success rate of the rat buccal submucosal fibrosis model was higher than moderate and high concentrations of arecoline,but the survival duration was significantly reduced after biopsy.Mechanical stimulati

关 键 词：槟榔碱 口腔黏膜下纤维化 机械刺激 颊黏膜 活检 SD大鼠 生存分析 

分 类 号：R78[医药卫生—口腔医学]