MMP-9抑制剂对口腔鳞癌细胞增殖和侵袭能力的影响  

Effect of MMP-9 inhibitor on oral squamous cell carcinoma

作　　者：尹盼盼 宿颖[1] 陈素红 付洁[2] 张辛燕[1] YIN Pan-pan;SU Ying;CHEN Su-hong;Fu Jie;ZHANG Xin-yan(Beijing Institute of Dental Research, Capital Medical University School of Stomatology, Beijing 100050, China)

机构地区：[1]首都医科大学口腔医学院口腔医学研究所,北京100050 [2]首都医科大学口腔医学院黏膜科,北京100050

出　　处：《北京口腔医学》2019年第3期121-125,共5页Beijing Journal of Stomatology

基　　金：国家自然科学基金(81772868)

摘　　要：目的 观察MMP-9抑制剂对人口腔鳞癌细胞系CAL27增殖和侵袭能力的影响。方法 选取人口腔鳞癌细胞系CAL27进行培养,用不同浓度的MMP-9抑制剂BB-94处理对数期CAL27细胞,通过RT-PCR检测BB-94在基因水平上对MMP-9的抑制效果。细胞免疫化学实验检测BB-94在蛋白水平上对MMP-9的抑制效果。MTT实验检测MMP-9抑制剂BB-94对人口腔鳞癌细胞增殖能力的影响。划痕实验检测MMP-9抑制剂BB-94对人口腔鳞癌细胞迁移能力的影响;Transwell实验检测MMP-9抑制剂BB-94对人口腔鳞癌细胞侵袭能力的影响。结果 MMP-9抑制剂能有效抑制人口腔鳞癌细胞CAL27的增殖、迁移和侵袭能力。结论 抑制MMP-9的表达可以抑制人口腔鳞癌细胞CAL27的增殖、迁移和侵袭等恶性生物学行为。Objective To observe the effect of MMP-9 inhibitor on human oral squamous cell carcinoma (OSCC) cell line CAL27. Methods OSCC cell line CAL27 was cultured in DMEM high glucose medium with 10% FBS and the logarithmic phase CAL27 cells were treated with different concentrations of MMP-9 inhibitor BB-94. The inhibiting effect of BB-94 on MMP-9 in the gene level was detected by RT-PCR assay. The inhibiting effect of BB-94 on MMP-9 in the protein level was detected by cell immunochemistry assay. The effect of BB-94 on the proliferation of human oral squamous cell carcinoma cells was detected by MTT assay. The effect of BB-94 on the migration ability of OSCC was examined by scratching assay. Transwell experiment examined the invasion ability of human oral squamous cell carcinoma. Results MMP-9 inhibitor could effectively inhibit the proliferation, migration and invasion of OSCC cells CAL27. Conclusion Inhibiting the expression of MMP-9 has a significant inhibitory effect on the malignant biological behavior of OSCC cells CAL27.

关 键 词：MMP-9 口腔鳞癌 抑制剂 

分 类 号：R739.8[医药卫生—肿瘤]