癌基因HuR调节胃癌功能的研究  

Effects of HuR on cell viability, migration and invasion of gastric cancer cell line MGC-803

作　　者：曹小梅 曹楠婧 王福花[2] 孙瑞芳[3] 李峰[2] 郭素堂[1,2] CAO Xiao-mei;CAO Nan-jing;WANG Fu-hua;SUN Rui-fang;LI Feng;GUO Su-tang(Department of Biochemistry and Molecular Biology, Basic Medical College, Shanxi Medical University, Taiyuan 030001, China;Department of Molecular Biology, Shanxi Provincial Cancer Institute, Taiyuan 030013, China;Sample Library, Shanxi Provincial Cancer Hospital, Taiyuan 030013, China)

机构地区：[1]山西医科大学生物化学与分子生物学教研室,山西太原030001 [2]山西省肿瘤研究所分子生物室,山西太原030013 [3]山西省肿瘤医院样本库,山西太原030013

出　　处：《中国病理生理杂志》2019年第7期1328-1332,共5页Chinese Journal of Pathophysiology

基　　金：山西省卫生和计划生育委员会科研基金资助项目(No.201601060);山西省重点研发计划项目(No.201803D31166);山西省肿瘤医院博士基金资助项目(No.2017A07)

摘　　要：目的:探讨癌基因HuR在胃癌组织的表达和对胃癌MGC-803细胞功能的影响。方法:使用RT-qPCR分析80例临床确诊的胃癌患者胃癌组织样本中HuR的表达水平;利用pSIH载体构建敲减HuR的重组质粒,实现HuR在MGC-803细胞中的低表达,以空载体pSIH作为对照;使用划痕实验检测细胞迁移能力,使用Transwell实验检测细胞侵袭能力,使用CCK-8法检测细胞活力。结果:RT-qPCR结果显示,与癌旁对照相比,67例(84%)胃癌组织中出现HuR的表达上调;低表达HuR能显著抑制MGC-803细胞的侵袭能力、迁移能力和活力(P<0.05),提示HuR作为癌基因起作用。结论:HuR的异常表达可能与胃癌的发生发展有关,低表达HuR可以抑制胃癌MGC-803细胞的侵袭、迁移和活力。AIM: To investigate the effects of HuR on cell function of gastric cancer cell line MGC-803. METHODS: The mRNA expression level of HuR was detected by RT-qPCR in the tumor samples of 80 gastric cancer patients diagnosed clinically. HuR gene knock-down was achieved by transfection of si-HuR into the MGC-803 cells. The invasion, migration and viability of MGC-803 cells were measured by the scratch wound hearing, Transwell and CCK-8 assays, respectively. RESULTS: High mRNA expression of HuR was observed in 67 cases(84%) of gastric cancer tissues as compared with their control samples. Furthermore, knock-down of HuR expression effectively inhibited the invasion, migration and viability of the MGC-803 cells(P<0.05), indicating that HuR play an important role in gastric cancer as an oncogene. CONCLUSION: Abnormal expression of HuR is correlated with the progression of gastric cancer. Knock-down of HuR expression inhibits the invasion, migration and viability of MGC-803 cells.

关 键 词：HuR基因 胃癌 细胞活力 细胞迁移 细胞侵袭 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]