The Susceptibility of Primary Dermis Fibroblasts from the Chinese Tree Shrew to Human Cytomegalovirus Infection  

作　　者：Shu-Wei Dong Ling-Shuai Jiao Ming Yang Ying-Liang Duan Yi-Bo Chen Fei Zhao A-Mei Zhang Li Liu Min-Hua Luo Xue-Shan Xia 

机构地区：[1]Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China [2]The Key Laboratory of Tropical and Subtropical Animal Viral Diseases in Yunnan Province,Kunming 650224,China [3]State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China

出　　处：《Virologica Sinica》2019年第3期270-277,共8页中国病毒学（英文版）

基　　金：supported by the National Key Research and Development Project of China (2017YFC1309302);the Applied Basic Research Projects of Yunnan Province (2018FD034);the Yunnan Provincial Department of Education Science Research Fund Project (1405189906)

摘　　要：As a universal pathogen leading to neonatal defects and transplant failure,human cytomegalovirus(HCMV)has strict species specificity and this has prevented the development of a suitable animal model for the pathogenesis study.The mechanism of cross-species barrier remains elusive and there are so far no non-human cell culture models that support HCMV replication.The Chinese tree shrew(Tupaia belangeri chinensis)is a small laboratory animal and evolutionary closely related with primates.We investigated the susceptibility of primary tree shrew dermis fibroblasts(TSDF)to HCMV infection.Infection with a GFP-expressing HCMV virus resulted in green fluorescence in infected cells with the expression of IE1,UL44 and pp28.The titers of cell-free viruses reached 10^3 PFU/mL at 96 hpi,compared to titers of 10^4 PFU/mL observed in primary human foreskin fibroblasts.Our results suggested that TSDF was semi-permissive for HCMV infection.The TSDF model could be further used to investigate key factors influencing cross-species multiplication of HCMV.As a universal pathogen leading to neonatal defects and transplant failure, human cytomegalovirus(HCMV) has strict species specificity and this has prevented the development of a suitable animal model for the pathogenesis study. The mechanism of cross-species barrier remains elusive and there are so far no non-human cell culture models that support HCMV replication. The Chinese tree shrew(Tupaia belangeri chinensis) is a small laboratory animal and evolutionary closely related with primates. We investigated the susceptibility of primary tree shrew dermis fibroblasts(TSDF) to HCMV infection. Infection with a GFP-expressing HCMV virus resulted in green fluorescence in infected cells with the expression of IE1, UL44 and pp28. The titers of cell-free viruses reached 103 PFU/mL at 96 hpi, compared to titers of 104 PFU/mL observed in primary human foreskin fibroblasts. Our results suggested that TSDF was semi-permissive for HCMV infection. The TSDF model could be further used to investigate key factors influencing cross-species multiplication of HCMV.

关 键 词：Human cytomegalovirus(HCMV) PRIMARY tree SHREW DERMIS fibroblasts(TSDF) CROSS-SPECIES infection Semi-permissiveness 

分 类 号：Q[生物学]