D-双功能蛋白在大鼠和裸鼠肝细胞癌生长中的作用  

Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice

作　　者：路欣 刘英 贾蕾 孔令玉[3] 刘殿卿[1] LU Xin;LIU Ying;JIA Lei(Department of Clinical Laboratory,Tangshan Maternal and Child Health Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山市妇幼保健院检验科,河北唐山063000 [2]华北理工大学附属医院,河北唐山063000 [3]河北医科大学,石家庄050017

出　　处：《临床肝胆病杂志》2019年第7期1532-1535,共4页Journal of Clinical Hepatology

基　　金：河北省2018年医学科学研究重点课题计划(20181317)

摘　　要：目的探讨肝细胞癌大鼠模型肝癌组织中D-双功能蛋白(DBP)的表达及DBP诱导裸鼠肿瘤生长情况。方法 22只雄性SD大鼠随机分为2组,正常对照组大鼠8只,腹腔注射2-乙基亚硝胺诱导肝细胞癌模型组大鼠14只,蛋白免疫印迹、免疫组化和逆转录聚合酶链反应检测DBP表达。14只雄性SPF级BALB/c-nu小鼠,随机分为2组,用空质粒和DBP过表达质粒分别转染HepG2细胞,将2组细胞分别注射于裸鼠皮下,空质粒对照组8只,DBP高表达组6只,检测2组裸鼠成瘤的大小。计量资料2组间比较采用t检验。结果肝细胞癌模型组大鼠肝癌组织中DBP的蛋白表达高于正常大鼠肝脏,差异具有统计学意义(1. 10±0. 35vs 0. 67±0. 12,t=-7. 48,P <0. 05);mRNA表达也高于正常大鼠肝脏,差异具有统计学意义(3. 70±0. 85 vs 1. 17±0. 72,t=-20. 46,P <0. 05)。DBP过表达组的裸鼠肿瘤体积显著大于空质粒组,差异具有统计学差异[(7590. 50±1867. 97) mm^3vs (1663. 78±420. 24)mm^3,t=-39. 78,P <0. 01]。结论高表达的DBP在大鼠肝癌的发展进程中发挥了促进作用,并且为肝癌的治疗和抑制药物的研发提供新的靶点。Objective To investigate the expression of D-bifunctional protein (DBP) in hepatocellular carcinoma (HCC) tissue in rats and the growth of DBP-induced HCC in nude mice. Methods A total of 22 male Sprague-Dawley rats were randomly divided into normal control group with 8 rats and model group with 14 rats treated with intraperitoneally injected diethylnitrosamine to induce HCC, and Western blotting, immunohistochemistry, and RT-PCR were used to measure the expression of DBP. A total of 14 specific pathogen-free male BALB/c-nu mice were randomly divided into two groups. HepG2 cells were transfected with empty plasmid or DBP overexpression plasmid and were then injected subcutaneously into nude mice. There were 8 mice in the empty plasmid control group and 6 mice in the DBP high-expression plasmid group, and tumor size was measured for both groups. The t -test was used for comparison of continuous data between groups. Results The rats with HCC had significantly higher protein and mRNA expression of DBP in liver tissue than normal rats (protein: 1.10±0.35 vs 0.67±0.12, t=-7.48, P <0.05;mRNA: 3.70±0.85 vs 1.17±0.72, t=-20.46, P <0.05). The DBP high-expression plasmid group had a significantly higher tumor volume than the empty plasmid group [(7590.50±1867.97)mm^3 vs (1663.78±420.24)mm^3, t =-39.78, P <0.01]. Conclusion Highly expressed DBP can promote the progression of HCC in rats and thus provides a new target for the treatment of HCC and the research and development of inhibitory drugs.

关 键 词：癌 肝细胞 D-双功能蛋白 大鼠 Sprague-Dawley 小鼠 近交BALB C 动物实验 

分 类 号：R735.7[医药卫生—肿瘤]