菌糠炭与微生物协同吸附-降解石油烃类污染物  

Adsorption and Degradation of Petroleum Hydrocarbon With Biochars and Microorganisms

作　　者：张博凡 徐文斐 王加华 熊鑫 韩卓[3] 张秀霞[1] 张钊 刘会娥[1] 顾莹莹 ZHANG Bofan;XU Wenfei;WANG Jiahua;XIONG Xin;HAN Zhuo;ZHANG Xiuxia;ZHANG Zhao;LIU Huie;GU Yingying(College of Chemical Engineering,China University of Petroleum,Qingdao 266580,China;Shanghai sus Environment Co.Ltd.,Shanghai 201700,China;Technical Detection Center of Shengli Oilfield Branch,Dongying 257000,China)

机构地区：[1]中国石油大学(华东)化学工程学院,山东青岛266580 [2]上海康恒环境股份有限公司,上海201700 [3]胜利油田分公司技术检测中心,山东东营257000

出　　处：《石油学报（石油加工）》2019年第4期736-743,共8页Acta Petrolei Sinica(Petroleum Processing Section)

基　　金：中国石油科技创新基金研究项目(2017D-5007-0601);山东省自然科学基金项目(ZR2014BM023);青岛市科技发展指导计划项目(KJZD-12-65-jch);中央高校基本科研业务费专项资金(14CX06101A)资助

摘　　要：以菌糠为原材料,在不同热解温度(250~650℃)下限氧热解制备菌糠炭,通过分析菌糠及菌糠炭结构的差异,探究其对微生物、石油烃的吸附性能及固定化菌株苍白杆菌Q1对石油烃的降解效果。结果表明:随着热解温度升高,菌糠炭对微生物吸附效果提高,其中550℃菌糠炭吸附固定化量最高为1.582×10^10CFU/g,SEM扫面电镜结果显示菌株主要吸附在材料表面。高温炭对石油烃吸附较好,其中550℃菌糠炭对胶质、沥青质吸附率最高,分别为36.33%、25.59%;吸附效果均与孔结构、芳香性相关显著,其协同微生物对石油烃四组分总体降解效率高,均优于其他热解温度下制备的菌糠炭组,pH值和有机碳含量对微生物吸附-降解影响较明显,550℃菌糠炭对微生物降解石油烃具有强化作用。Biochar samples were prepared at different pyrolysis temperatures (250-650 ℃) using spent mushroom substrate (SMS) as raw materials.Effect of different biochar structures on adsorption rate of Ochrobactrum sp.Q1,oil and degradation rate were analyzed.Experimental results indicated that 550 ℃-biochar (BC550) has the best adsorption performance (i.e.,1.582×10^10 CFU/g) on microorganism.SEM analyses showed that most microorganisms were immobilized on the surface of the materials.BC550 also has the best adsorption performance on resin and asphaltenes,which were 36.33% and 25.59%,respectively.It was found that adsorption performance is strongly related with pore structure and aromaticity.BC550 immobilized bacteria have better degradation rate for different oil components (i.e.,saturates,aromatics,resins and asphaltenes) than other biochar groups prepared at other different pyrolysis temperatures.In addition,pH value and organic carbon content have obvious effect on microbial adsorption-degradation rate.BC550 has enhanced effect on microbial degradation of petroleum hydrocarbons.

关 键 词：菌糠 生物炭 吸附 石油烃降解率 

分 类 号：X131[环境科学与工程—环境科学] X712