抑制Wnt/β-catenin信号通路对上唇发育的影响  

Effect of Inhibition of Wnt/β-catenin Signaling Pathway on Development of Upper Lip

作　　者：李智聪 邹瑞[1] Hye-Jin Tak Sang-Hwy Lee 朴正国[1] LI Zhicong;ZOU Rui;PIAO Zhengguo(Key Laboratory of Oral Medicine,School and Hospital of Stomatology,Guangzhou Medical University,Guangzhou 510140,China;Department of Applied Life Science,College of Dentistry,Yonsei University.Seoul,Korea)

机构地区：[1]广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室,广东广州510140 [2]Oral Science Research Center,College of Dentistry,Yonsei University,Seoul,South Korea [3]Department of Oral and Maxillofacial Surgery,College of Dentistry,Yonsei University, 50 Yonsei-ro,Seodaemun-gu,Seoul03722,South Korea

出　　处：《口腔医学研究》2019年第7期695-700,共6页Journal of Oral Science Research

基　　金：广东省科技计划项目(编号:2012B032000012、2017A020215141)

摘　　要：目的:探讨通过抑制Wnt/β-catenin信号表达对上唇发育的影响。方法:通过在H-H22阶段的鸡胚上颌突植入浸有XAV939、DMSO的微珠以及只作切口不植入微珠,构建实验组、对照组和假实验组,并继续孵育至H-H38阶段,体视显微镜以及mirco-CT观察并记录胚胎上唇的发育情况。结果:Wnt/β-catenin信号受抑制后,H-H28阶段的鸡胚植入侧上颌突与中鼻突和侧鼻突的融合延迟,H-H38阶段的鸡胚植入侧的上唇出现发育延缓,面中线偏向植入侧;实验组的正常侧、对照组以及假实验组的双侧上唇发育未受影响。结论:在鸡胚上唇发育过程中,通过抑制Wnt/β-catenin信号表达,可延迟上颌突和中鼻突的融合,使上唇发育延迟,进而导致上颌骨发育不足或畸形。Objective: To investigate the effect of inhibiting the Wnt/β-catenin signal on the growth of upper lip.Methods: The maxillary prominence of chicken embryo was implanted the beads impregnated with XAV939 and DMSO and implanted without beads into in the H-H22 stage.The experimental group,control group,and sham group were constructed separately.The incubation was continued until the H-H38 stage.The development of the upper lip of the embryo was observed and recorded by stereomicroscopy and micro-CT.Results: After the Wnt/β-catenin signal was inhibited,the fusion of the maxillary prominence and the frontonasal mass and the lateral nasal prominence of the chicken embryo implanted at the H-H28 stage was delayed,and the upper lip of the chicken embryo implanted-side of the H-H38 stage was delayed in development.The midline was biased toward the implanted side.In addition,the development of the bilateral upper lip of the normal side of the experimental group,the control group,and the sham group was not affected.Conclusion: In the development of the upper lip of the chicken embryo,by inhibition of Wnt/β-catenin signal,the fusion of the maxillary prominence,and the frontonasal mass can be delayed so that the development of the upper lip was delayed,leading to the insufficient development or deformity of maxilla bone.

关 键 词：WNT/Β-CATENIN信号通路 鸡胚 上唇发育 

分 类 号：R782.2[医药卫生—口腔医学]