半育耳蕨化学成分及其抑制α-葡萄糖苷酶活性研究  

Chemical constituents and α-glucosidase inhibitory activities of Polystichum semifertile

作　　者：陆怀 孙静贤[1] 蒋孟圆[1] 高利斌[1] 陈毅坚[1] 李育逵 黄相中[1] 田凯[1] 王韦[1] LU Huai;SUN Jing-xian;JIANG Meng-yuan;GAO Li-bin;CHEN Yi-jian;LI Yu-kui;HUANG Xiang-zhong;TIAN Kai;WANG Wei(Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education of China, Yunnan Minzu University, Kunming 650500, China)

机构地区：[1]云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室

出　　处：《云南民族大学学报（自然科学版）》2019年第4期320-325,共6页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(81460535);云南省应用基础研究项目(2017FD122)

摘　　要：采用MCI柱色谱、硅胶柱色谱、Sephadex LH-20凝胶柱色谱和半制备HPLC等色谱分离手段对半育耳蕨的化学成分进行研究.利用理化性质及波谱数据(MS、NMR)对其结构进行鉴定,从半育耳蕨体积分数95%的乙醇提取物中分离并鉴定了10个化合物,分别是2 R,3R-3,5,6,7,8,4′-hexahydroxyflavane(1)、(-)-表儿茶素(2)、(+)-epicatechin(3)、arachniodesin A(4)、4β-carboxymethyl-(-)-epicatechin methyl ester(5)、eriodictyol(6)、daucosterol(7)、3,4-二羟基苯甲酸乙酯(8)、圣草酚7-O-β-D-(6′-甲酯基)-吡喃葡萄糖醛酸苷(9)、圣草酚7-O-β-D-(6′-乙酯基)-吡喃葡萄糖醛酸苷(10).化合物1~10均为首次从耳蕨属中分离得到.活性测试结果表明,化合物4、5和6对α-葡萄糖苷酶具有较好的抑制活性,其IC 50分别为(26.2±1.9)、(32.6±2.5)和(51.5±3.5)μmol·L^-1.Ten compounds were isolated from the 95%ethanol extract of Polystichum semifertile by means of MCI gel,silica gel,sephadex LH-20 column chromatographies and semi-preparative HPLC.On the basis of physicochemical properties and spectroscopic data,their structures were identified as 2 R,3 R-3,5,6,7,8,4′-hexahydroxyflavane(1),(-)-epicatechin(2),(+)-epicatechin(3),arachniodesin A(4),4β-carboxymethyl-(-)-epicatechin methyl ester(5),eriodictyol(6),daucosterol(7),3,4-dihydroxybenzoic acid ethyl ester(8),eriodictyol 7-O-β-D-(6′-methyl ester)-glucuronpyranoside(9)and eriodictyol 7-O-β-D-(6′-ethyl ester)-pyrannoglucuronide(10).Compounds 1-10 were obtained from the genus Polystichum for the first time.Biological activity results showed that compounds 4,5 and 6 exhibitedα-glucosidase inhibitory activity with IC 50 values of(26.2±3.9),(32.6±2.5)and(51.5±3.6)μmol·L^-1.

关 键 词：耳蕨属 半育耳蕨 化学成分 α-葡萄糖苷酶抑制活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]