黄瓜生产过程中致病菌调查及耐药性分析  

Investigation of Pathogenic Bacteria and Drug Resistance Analysis in Cucumber Production Process

作　　者：邬元娟 张露月[3] 王文博 郭栋梁 郝艳芳[4] 刘磊 WU Yuan-juan;ZHANG Lu-yue;WANG Wen-bo(Institute of Agricultural Standards and Testing Technology for Agri-Products Shandong Academy of Agricultural Sciences & Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety,Jinan,Shandong 250100;NCSU - SAAS Joint Laboratory for Testing Technology & Risk Assessment for Agro-products,Jinan,Shandong 250100;Yantai Food and Drugs Inspection and Testing Center,Yantai,Shandong 264000)

机构地区：[1]山东省农业科学院农业质量标准与检测技术研究所,山东省食品质量与安全检测技术重点实验室,山东济南250100 [2]山东省农业科学院-北卡罗莱纳州立大学农产品检测技术和风险评估联合实验室,山东济南250100 [3]烟台市食品药品检验检测中心,山东烟台264000 [4]北京勤邦生物技术有限公司,北京102206 [5]北京良润生物科技有限公司,北京102200

出　　处：《安徽农业科学》2019年第14期207-209,共3页Journal of Anhui Agricultural Sciences

基　　金：国家农产品质量安全风险评估重大专项(GJFP201801304);山东省农业科学院农业科技创新工程项目(CXGC2018E05)

摘　　要：对黄瓜生产过程中黄瓜、土壤、基肥、灌溉用水及植株中感染沙门氏菌、金黄色葡萄球菌、大肠杆菌、单增李斯特氏菌的情况进行调查,结果表明,共计102批次样本中黄瓜样品、植株及土壤中各检出一株大肠杆菌,植株中检出一株金黄色葡萄球菌。对大肠杆菌及金黄色葡萄球菌进行8大类13种抗生素的药敏试验,结果表明,土壤中大肠杆菌为敏感菌株且对13种抗生素均敏感,植株中大肠杆菌仅对氨曲南耐药,黄瓜中大肠杆菌对氨苄西林、哌拉西林、氯霉素和四环素耐药,为多重耐药菌株;植株中金黄色葡萄球菌对青霉素、红霉素、克林霉素及磺胺甲噁唑耐药,为多重耐药菌株。Cucumber, soil, base fertilizer, irrigation water and plant infected with Salmonella, Staphylococcus aureus, Escherichia coli and Listeria monocytogenes during the production of cucumber were investigated.The results showed that among the 102 tested samples, Escherichia coli was detected in one ripe cucumber, one cucumber plant and one cultivated soil sample respectively,and Staphylococcus aureus was found in one cucumber plant sample.The susceptibility test of 8 major classes of 13 types of antibiotics against Escherichia coli and Staphylococcus aureus showed that the Escherichia coli in cultivated soil was sensitive strains, which was susceptible to the 13 types of antibiotics. Escherichia coli in cucumber plants was resistant to aztreonam only;Escherichia coli in ripe cucumber was resistant to ampicillin, piperacillin, chloramphenicol and tetracycline, making ripe cucumber multi-drug-resistant strains. Staphylococcus aureus in cucumber plants was resistant to penicillin, erythromycin, clindamycin and sulfamethoxazole, which made cucumber plants multi-drug-resistant strains.

关 键 词：黄瓜 大肠杆菌 金黄色葡萄球菌 耐药性 

分 类 号：S41-30[农业科学—植物保护]