斑点追踪成像评价脓毒症大鼠早期心肌损伤及柚皮苷预处理效果  

Speckle tracking imaging in evaluation of early myocardial injury of sepsis rats and effect of naringin pretreatment

作　　者：孙立娟 任卫东[1] 乔伟[1] 肖杨杰[1] 崔莉[1] SUN Lijuan;REN Weidong;QIAO Wei;XIAO Yangjie;CUI Li(Department of Ultrasound,Shengjing Hospital of China Medical University,Shenyang 110004,China;Department of Ultrasound,the First Hospital of Qinhuangdao,Qinhuangdao 066000,China)

机构地区：[1]中国医科大学附属盛京医院超声科,辽宁沈阳110004 [2]秦皇岛市第一医院超声科,河北秦皇岛066000

出　　处：《中国医学影像技术》2019年第7期961-965,共5页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(85171686)

摘　　要：目的探讨斑点追踪成像(STI)技术评价脂多糖(LPS)诱导脓毒症大鼠早期心肌损伤及柚皮苷(Nar)预处理对逆转心肌损伤效果的应用价值。方法将36只SD大鼠随机分为LPS+Nar 50组(n=9)、LPS+Nar 100组(n=9)、LPS组(n=10)及对照组(n=8)。对LPS+Nar 50组、LPS+Nar100组分别以50、100 mg/kg体质量灌胃给予Nar混悬液,对照组、LPS组大鼠给予等量生理盐水,连续灌胃7天;于最后一次灌胃结束后1 h,LPS+Nar50组、LPS+Nar 100组及LPS组腹腔注射5 mg/kg体质量LPS,对照组给予等量生理盐水。于LPS注射6 h后检测4组大鼠左心室射血分数(LVEF)及Tei指数,左心室各节段圆周应变(SC)峰值,左心室整体心内膜下心肌、中间心肌层及心外膜下心肌SC峰值(分别记为GSCendo、GSCmid、GSCepi),收缩期圆周应变率(SrC)峰值(SrC S)、舒张早期SrC峰值(SrC E)、舒张晚期SrC峰值(SrC A);检测血清肌酸激酶(CK)、乳酸脱氢酶(LDH)水平,以HE染色观察心肌组织病理改变。结果 LPS组左心室各节段SC峰值均低于对照组和LPS+Nar 100组(P均<0.05);LPS+Nar 50组前间隔、前壁、下壁及后间隔SC峰值均高于LPS组而低于LPS+Nar 100组;LPS+Nar 50组侧壁SC峰值低于LPS+Nar 100组(P均<0.05)。LPS组LVEF、GSCendo、GSCmid、SrC S、SrC E及SrC A均低于其余3组,Tei指数、CK及LDH均高于其余3组(P均<0.05);LPS+Nar 50组LVEF、GSCmid、SrC S、SrC E及SrC A均低于LPS+Nar 100组,Tei指数、CK及LDH均高于LPS+Nar 100组(P均<0.05)。病理结果显示LPS组大鼠心肌组织部分细胞核固缩,充血,炎症细胞增多;LPS+Nar 50组、LPS+Nar 100组上述病理改变较LPS组减轻,以LPS+Nar 100组为著。结论 STI技术可用于评价LPS诱导脓毒症大鼠早期心肌损伤及Nar预处理减轻心肌损伤的效果。Objective To investigate the value of speckle tracking imaging (STI) technique in evaluating lipopolysaccharide (LPS) induced early myocardial injury in sepsis rats and naringin (Nar) pretreatment in reversing myocardial injury.Methods Thirty-six SD rats were randomly divided into LPS+Nar 50 group (n=9),LPS+Nar 100 group (n=9),LPS group (n=10) and control group (n=8).Rats in LPS+Nar 50 group and LPS+Nar 100 group were given Nar suspension by gavage with 50,100 mg/kg,respectively,while in other two groups were given the same amount of normal saline.Continuous gavage was performed for 7 days,then rats in LPS +Nar 50 group,LPS+Nar 100 group and LPS group were intraperitoneally injected with 5 mg/kg LPS 1 hour after the last gavage,while in control group were given the same amount of normal saline.Six hours after LPS injection,all rats were tested for left ventricular ejection fraction (LVEF) and Tei index,peak circumferential strain (SC) at each segment of the left ventricle (LV),LV global subendocardial,middle and subepicardial SC (respectively for GSCendo,GSCmid,GSCepi),systolic peak rate of SC (SrC S),peak early diastolic SC rate (SrC E),late diastolic peak rate of SC (SrC A).Serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) were detected,and pathological changes of myocardial tissue were observed by HE.Results The peaks of SC at each segment of LV in LPS group were lower than those in control group and LPS+Nar 100 group (all P<0.05).In LPS+Nar 50 group,SC peaks of anterior septum,anterior wall,inferior wall and posterior septum were higher than those in LPS group but lower than those in LPS+Nar 100 group: SC peak of lateral wall in LPS+Nar 50 group was lower than that in LPS+Nar 100 group (all P< 0.05).LVEF,GSCendo,GSCmid,SrC S,SrC E and SrC A in LPS group were significantly lower than the other three groups,and Tei index,CK and LDH were all higher than in the other three groups (all P< 0.05).LVEF,GSCmid,SrC S,SrC E and SrC A in LPS+Nar 50 group were lower than those in LPS+Nar 100 group,while

关 键 词：心肌疾病 脓毒症 脂多糖类 心室功能 左 斑点追踪成像 超声检查 

分 类 号：R-322[医药卫生] R631.2