芦笋老茎皂苷对糖尿病大鼠的降血糖作用研究  

THE HYPOGLYCAEMIC ACTIVITY OF SAPONINS FROM ASPARAGUS OFFICINALIS L. BY-PRODUCTS IN DIABETIC RATS

作　　者：朱兴磊 张雯[3] 高云 张国庆 ZHUXing-lei;ZHANG Wen;GAOYun;ZHANG Guo-qing(Editorial Department of Journal of Yunnan Normal University, Yunnan Normal University, Kunming 650092;School of life science, YunnanNormal University, Kunming 650092;School of life science, East China Normal University, Shanghai 200241, China)

机构地区：[1]云南师范大学学报编辑部,云南昆明650092 [2]云南师范大学生命科学学院,云南昆明650092 [3]华东师范大学生命科学学院,上海200241

出　　处：《营养学报》2019年第3期270-275,共6页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.3167011258);云南省教育厅科学研究基金(No.2019J0070);云南师范大学博士科研启动基金

摘　　要：目的探究芦笋老茎皂苷(saponins from Asparagus officinalis L. by-products, SAO)对链脲佐菌素(STZ)诱导的类似1型糖尿病大鼠的降血糖作用。方法雄性SD大鼠90只,随机分为:正常对照(NC,蒸馏水)组9只和糖尿病诱导组81只。糖尿病诱导组大鼠一次性腹腔注射47 mg/kg·bw剂量的STZ溶液诱发糖尿病,并将诱导成型的糖尿病大鼠随机分为糖尿病模型(DC,蒸馏水)、格列本脲(Gli, 10 mg/(kg·d))、SAO低剂量(SAO-L, 20 mg/(kg·d))、SAO中剂量(SAO-M, 40 mg/(kg·d))和SAO高剂量(SAO-H, 80 mg/(kg·d))5组,每组9只,连续灌胃相应药物或蒸馏水21 d。测量大鼠体质量、采食量和饮水量,空腹血糖值、血清总胆固醇、甘油三酯及肝糖原按试剂盒说明书方法测定,血清胰岛素采用双抗体放射免疫法测定,并于给药7 d时进行口服糖耐量实验。结果与DC组大鼠相比,灌喂不同剂量SAO的大鼠体质量下降明显得到抑制(P <0.05),空腹血糖值有不同程度的降低,SAO-M组大鼠的餐后血糖值在30、60和120 min均有极显著降低(P <0.01),SAO-H组大鼠的餐后血糖值在30 min时也有显著降低(P <0.05),SAO三个剂量组大鼠的糖耐量曲线下面积都明显减少(P <0.05);此外,SAO-M组大鼠的血清胰岛素和肝糖原含量显著增加(P <0.05)。结论 SAO能够明显降低糖尿病大鼠的血糖水平,且SAO-M的治疗效果最好。Objective To study the hypoglycaemic activity of saponins from Asparagus officinalis L. by-products (SAO) in streptozotocin (STZ)-induced type 1-like diabetic rats. Methods Male Sprague-Dawley rats were randomly divided into normal control (NC) group and STZ-induced diabetic group. Diabetes was induced in rats by a single intraperitoneal injection of 47 mg kgH body weight (BW) of STZ. Then diabetic rats were divided into diabetic control (DC, distilled water) group, glibenclamide (Gli, 10 mg/(kg d)) group, low-dose SAO (SAO-L, 20 mg/(kg d)) group, medium-dose SAO (SAO-M, 40 mg/(kg d)) group and high-dose SAO (SAO-H, 80 mg/(kg d)) group. The drugs or vehicles were orally administered to rats once a day for 21 days. Body weight, food and water consumption were measured. Fasting serum glucose, serum triglyceride, total cholesterol and hepatic glycogen levels were determined using commercial kits. Serum insulin was measured by a double-antibody radioimmunoassay(RIA) method. Furthermore, an oral glucose tolerance test was performed. Results Compared with DC group, administration of SAO significantly decreased fasting serum glucose while markedly increased body weight in diabetic rats. In oral glucose tolerance test, SAO treatment prevented the blood glucose level from rising, especially in SAO-M group. SAO-M group showed significant decreased blood glucose levels at 30, 60 and 120 min (P<0.01) and SAO-H group showed a reduction at 30 min (P<0.05).The area under the glucose curve was reduced significantly in all SAO-treat groups (P<0.05). Furthermore, lower serum insulin level and hepatic glycogen level were found in SAO-M group (P<0.05). Conclusion The results of this study demonstrate that SAO has hypoglycaemic activity.

关 键 词：芦笋 皂苷 糖尿病大鼠 降血糖 链脲佐菌素 

分 类 号：Q946.8[生物学—植物学] R589.2[医药卫生—内分泌]