大蒜素对重症中暑大鼠炎性介质和氧化应激及脏器功能的影响  

Effects of allicin on inflammatory mediators, oxidative stress and organ function in rats with severe heat stroke

作　　者：沈德新 李庆华 孙荣青[3] 吴籽欣 王海伟 董桂云 王楠楠 闫进 SHEN Dexin;LI Qinghua;SUN Rongqing;WU Zixin;WANG Haiwei;DONG Guiyun;WANG Nannan;YAN Jin(Department of Pediatrics, the 988th Hospital of PLA, He′nan Province, Zhengzhou 450042, China;Department of Intensive Care Unit, the 990th Hospital of PLA, He′nan Province, Zhumadian 463008, China;Department of Intensive Care Unit, the First Affiliated Hospital of Zhengzhou University, He′nan Province, Zhengzhou 450042, China;Department of Education and Training Program for Excellent Doctors in Clinical Medicine, Xinxiang Medical College, He′nan Province, Xinxiang 453000, China)

机构地区：[1]解放军第九八八医院儿科,河南郑州450042 [2]解放军第九九零医院重症医学科,河南驻马店463008 [3]郑州大学第一附属医院重症医学科,河南郑州450052 [4]新乡医学院临床医学卓越医生教育培养计划,河南新乡453000

出　　处：《中国医药导报》2019年第19期25-28,共4页China Medical Herald

基　　金：济南军区后勤科研计划项目(JN11L047)

摘　　要：目的观察大蒜素对重症中暑大鼠炎性因子水平、抗氧化及脏器功能的影响。方法将60只雄性SD大鼠按随机数字表法分为正常对照组(n=20)和热打击组(n=40),将热打击组40只大鼠随机分为生理盐水组和大蒜素组,每组20只。实验开始前热打击组所有大鼠均接受环境模拟的适应性训练2周。并与适应性训练最后10 d,大蒜素组每日定时经口腔灌胃大蒜素20 mg/kg,生理盐水组给予相同体重比例的生理盐水。适应性训练结束后将热打击组大鼠置于仿真热气候动物舱进行热打击,时间为90 min。出舱24 h所有大鼠抽取股动脉血检测血清白细胞介素17(IL-17)、白细胞介素1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、血清超敏-C反应蛋白(hs-CRP)、丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、晚期蛋白氧化产物(AOPPs);应用全自动生化分析仪检测血肌酐(SCr)、尿素氮(BUN)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)含量。结果与正常对照组比较,经热打击及运动处理后,大蒜素组及生理盐水组大鼠血清IL-17、IL-1β、TNF-α、hs-CRP及MDA、AOPPs均显著升高,SOD、T-AOCf均显著下降,肝、肾生化指标SCr、BUN、ALT、AST水平均显著升高,差异均有高度统计学意义(P <0.01);与生理盐水组比较,大蒜素组大鼠血清IL-17、IL-1β、TNF-α、hs-CRP及MDA、AOPPs显著降低,SOD、T-AOC显著升高,血清SCr、BUN、ALT、AST含量水平显著降低,差异均有高度统计学意义(P <0.01)。结论热打击及运动处理后,大鼠血清炎性因子产生和释放增加,脂质过氧化水平提高。大蒜素可降低重症中暑大鼠血清炎性因子的产生和释放及脂质过氧化水平,达到保护脏器功能的作用。Objective To observe the effect of allicin on the levels of inflammatory factors, antioxidation and organ function in rats with severe heat stroke. Methods Sixty male SD rats were randomly divided into normal control group (n = 20) and heat shock group (n = 40). Forty rats in heat shock group were randomly divided into saline group and allicin group, with 20 rats in each group. All rats in the heat shock group received adaptive training of environmental simulation for 2 weeks before the start of the experiment. At the last 10 d of adaptive training, the allicin group was given 20 mg/kg of allicin orally at regular intervals every day, and the saline group was given saline with the same proportion of body weight. After adaptive training, rats in the heat shock group were placed in the simulated hot climate animal cabin for 90 minutes. Femoral artery blood was taken from all rats 24 h after cabin exit to detect serum interleukin-17 (IL-17), interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), serum hs-CRP, malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and advanced protein oxidant products (AOPPs). Serum creatinine (SCr), blood urea nitrogen (BUN), alanine aminotransferase (ALT) and aspartate aminotrans ferase (AST) were measured by automatic biochemical analyzer. Results Compared with the normal control group, the levels of IL-17, IL-1β, TNF-α and hs-CRP in serum of rats in allicin group and saline group were significantly increased after heat shock and exercise treatment, the lipid peroxide MDA and AOPPs increased significantly, while the antioxidant SOD and T-AOC decreased significantly, and the levels of liver and kidney biochemical indexes SCr, BUN, ALT and AST were significantly increased, the differences were statistically significant (P < 0.01). However, compared with the saline group, the levels of IL-17, IL-1β, TNF-α, hs-CRP, MDA and AOPPs in serum of rats in allicin group were significantly decreased, the SOD and T-AOC increased significantly, the levels

关 键 词：大蒜素 重症中暑 氧化应激 炎性因子 脏器功能 大鼠 

分 类 号：R282.740[医药卫生—中药学]