microRNA-194及microRNA-497在结直肠癌患者血清中的表达和临床意义  

Expression and clinical value of serum microRNA-194 and microRNA-497 in colorectal cancer

作　　者：徐菲菲 陈昭华[1,3] 王亚南 XU Feifei;CHEN Zhaohua;WANG Ya′nan(Department of Clinical Laboratory,Suzhou Municipal Hospital,Suzhou,Jiangsu 215002,China;Changshu Medicine Examination Institute,Suzhou,Jiangsu 215500,China;Department of Clinical Laboratory,the Affiliated Suzhou Hospital of Nanjing Medical University,Suzhou,Jiangsu 215002,China)

机构地区：[1]苏州市立医院检验科,江苏苏州215002 [2]常熟市医学检验所,江苏苏州215500 [3]南京医科大学附属苏州医院检验科,江苏苏州215002

出　　处：《检验医学与临床》2019年第14期1997-2000,共4页Laboratory Medicine and Clinic

基　　金：江苏省苏州市“科教兴卫”青年科技项目(KJXW2014017)

摘　　要：目的探讨microRNA-194(miR-194)、microRNA-497(miR-497)在结直肠癌(CRC)患者血清中的表达及临床意义。方法收集52例CRC患者(CRC组)和22例健康体检者(对照组)血清标本,采用实时荧光定量聚合酶链反应(RT-PCR)检测miR-194、miR-497的表达量。结果 CRC组血清中miR-194表达为1.90(0.86~3.35),明显高于对照组[0.76(0.69~0.93)],CRC组血清中miR-497表达为1.57(0.62~2.38),高于对照组[0.79(0.36~1.15)],差异均有统计学意义(P<0.05)。不同年龄、肿块大小、肿瘤分化程度、淋巴结转移情况及TNM分期的CRC患者miR-194、miR-497水平比较,差异均无统计学意义(P>0.05)。结论 miR-194和miR-497在CRC患者血清中表达上升,其异常表达可能与CRC的发生有关。Objective To explore the expression level and clinical value of serum microRNA-194 (miR-194),microRNA-497 (miR-497) in colorectal cancer patients.Methods The expression levels of serum miR-194,miR-497 of 52 colorectal cancer patients (colorectal cancer group) and 22 healthy controls (control group) were determined by real-time quantitative PCR and compared.Results The relative expression level of serum miR-194 was 1.90(0.86-3.35) in colorectal cancer group,which was higher than 0.76(0.69- 0.93) in control group,the relative expression level of serum miR-497 was 1.57(0.62-2.38) in colorectal cancer group,which was higher than 0.79(0.36-1.15) in control group,the differences were significant (P<0.05).However,the expression levels of miR-194 and miR-497 had no significant differences in patients with different age,tumor size,differentiation degree and TNM stage (P>0.05).Conclusion miR-194 and miR-497 highly expressed in the serum of colorectal cancer patients,the abnormal expression might related to the occurrence.

关 键 词：结直肠癌 microRNA-194 microRNA-497 实时荧光定量聚合酶链反应 

分 类 号：R735.35[医药卫生—肿瘤]