邻苯二甲酸二甲酯对荧光假单胞菌的损伤研究  

Study on the damage caused by dimethyl phthalate on Pseudomonas fluorescens

作　　者：郭茹鑫 王志刚[1] 王春龙[1] 由义敏 王恒煦 GUO Ru-xin;WANG Zhi-gang;WANG Chun-long;YOU Yi-min;WANG Heng-xu(College of Life Science and Agroforestry,Qiqihar University,Qiqihar 161006,China;College of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]上海交通大学农业与生物学院,上海200240

出　　处：《农业环境科学学报》2019年第7期1476-1481,共6页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(31870493,31670375)~~

摘　　要：为探究邻苯二甲酸二甲酯(Dimethyl phthalate,DMP)对细菌的毒理作用,选择荧光假单胞菌(Pseudomonas fluorescens)为受试菌株,采用平板计数试验、对数生长数学模型、细菌表面Zeta电位、荧光偏振法、透射电镜和超高效液相色谱法,测定了P.fluorescens的比生长速率、代时、细胞表面电势、细胞膜流动性、细胞损伤程度和DMP在细胞内外分布,研究DMP对P.fluorescens的细胞毒性。结果表明:经0、10、20、40 mg·L^-1的DMP暴露后,细菌比生长速率减小,而代时增加;细菌表面Zeta电位从-12.21±0.56mV逐渐降低为-18.13±0.67 mV,同时DMP增加了细菌细胞膜的流动性,导致细胞明显变形、质壁分离严重并伴有内容物的泄露;在细胞内,DMP累积浓度从0.11±0.10 mg·L-1增加到6.78±0.25 mg·L^-1;在细胞外,DMP累积浓度从8.44±0.37 mg·L^-1增加到30.52±0.88 mg·L^-1。研究表明,DMP破坏了P.fluorescens的细胞壁和细胞膜,影响了细菌正常生长。Dimethyl phthalate(DMP)is a ubiquitous pollutant that is very harmful to microorganisms.Pseudomonas fluorescens(P.fluorescens)is one of the most important beneficial bacteria in the environment.To explore the toxicological effects of DMP on bacteria,P.fluorescens was selected and investigated using several bio-techniques,such as the polarization method,transmission electron microscopy,and ultra-high performance liquid chromatography.The results showed that the specific growth rate of P.fluorescens was slower,but the generation time was extended under the 10,20 mg·L-1,and 40 mg·L-1DMP treatments.The zeta potential on the surface of P.fluorescens decreased gradually from-12.21±0.56 mV to-18.13±0.67 mV under the 0,10,20 mg·L-1,and 40 mg·L-1DMP treatments.Meanwhile,the mobility of the cell membrane increased by the DMP treatment and resulted in obvious cell deformation,severe plasmolysis,and leakage of dissoluble matter.The cumulative concentration of DMP inside the cells increased from 0.11±0.10 mg·L-1to 6.78±0.25 mg·L-1,and the cumulative concentration outside the cells increased from 8.44±0.37 mg·L-1to 30.52±0.88 mg·L-1,under the 10,20 mg·L-1,and 40 mg·L-1DMP treatments.Therefore,we conclude that DMP destroys the cell wall and the cell membrane of P.fluorescens and strongly affects the normal growth of P.fluorescens.

关 键 词：邻苯二甲酸二甲酯 荧光假单胞菌 膜损伤 内容物泄露 

分 类 号：X171.5[环境科学与工程—环境科学]