HSP70-2基因多态性与急性胰腺炎的相关性分析  

Analysis of the correlation between HSP70-2 gene polymorphism and acute pancreatitis

作　　者：杨彩芳 闫乐[2] 梁浩卫 Yang Caifang;Yan Le;Liang Haowei(Henan xinmi hospital digestive internal medicine Henan new ones 452370;henan university first affiliated hospital of traditional Chinese medicine Henan zhengzhou 450000.)

机构地区：[1]新密市中医院消化内科,河南新密452370 [2]河南中医药大学第一附属医院,河南郑州450000

出　　处：《实验与检验医学》2019年第4期578-581,共4页Experimental and Laboratory Medicine

摘　　要：目的探讨热休克蛋白(HSP)70-2基因多态性与急性胰腺炎的相关性。方法选取2016年3月至2017年7月在河南省新密市中医院治疗的急性胰腺炎患者91例,其中轻型胰腺炎患者47例(轻型组),重型胰腺炎44例(重型组),同时选取健康志愿者100例作为对照组,采用聚合酶链反应-限制性片段长度多态性检测HSP70-2基因多态性;同时比较不同预后患者基因多态性的等指标差异。结果重型组基因型GG和等位基因G比例分别为43.18%和62.50%,明显高于轻型组和对照组(P<0.05);轻型组基因型GG和等位基因G比例分别为31.91%和52.13%,明显高于对照组(P<0.05);91例急性胰腺炎患者中死亡16例(死亡组);死亡组基因型GG比例为87.50%,明显高于存活组(P<0.05);死亡组C反应蛋白(CRP)、总胆固醇(TC)、甘油三酯(TG)、CT严重指(CTSI)评分和性生理与慢性健康评分(APACHEⅡ)评分明显高于存活组(P<0.05);Logistic回归分析,结果显示CTSI评分、APACHEⅡ评分和HSP70-2基因型GG是急性胰腺炎患者死亡的影响因素(OR=2.252(1.131~8.821)、2.188(1.201~6.984)和1.324(1.131~3.154),P<0.05)。结论HSP70-2基因GG型及等位基因G与急性胰腺炎病情、预后有关。Objective To investigate the correlation between the polymorphism of heat shock protein (HSP) 70-2 gene and acute pancreatitis. Methods From March 2016 to July 2017,91 cases of acute pancreatitis in Henan xinmi hospital were selected,including 47 cases of mild pancreatitis,44 cases of severe pancreatitis,and 100 cases of healthy volunteers were selected as control group,HSP70-2 gene polymorphism was detected by polymerase chain reaction restriction fragment length polymorphism;the difference of gene polymorphism and others indexwas compared between the patients with different prognosis. Results The proportion of genotype GG and allele G in severe group were 43.18% and 62.50%,which were significantly higher than that in mild group and control group(P<0.05);The proportion of genotype GG and allele G in mild group were 31.91% and 52.13%,which were significantly higher than that in control group (P<0.05);Of 91 cases of acute pancreatitis,16 cases died (death group);The proportion of genotype GG in the death group was 87.50%,which was significantly higher than that in the survival group(P<0.05);The CRP,TC,TG,CTSI score and APACHE II score in the death group were significantly higher than those in the survival group (P<0.05);Logistic regression analysis showed that CTSI score,APACHE II score and HSP70-2 genotype GG were the influencing factors of death in patients with acute pancreatitis (OR=2.252(1.131~8.82),2.188(1.201~6.984) and 1.324(1.131~3.154),P<0.05). Conclusion The GG and allele G of HSP70-2 gene are related to acute pancreatitis condition and prognosis.

关 键 词：HSP70-2基因 基因多态性 急性胰腺炎 病情程度 预后 

分 类 号：R576.R446[医药卫生—消化系统]