CREB1、BDNF基因多态性与河南汉族老年男性卒中后抑郁的关联性分析  

Correlation analysis between gene polymorphism of cyclic adenosine monophosphate response element-binding protein 1,brain-derived neurotrophic factor and post-stroke depression in older males of Henan Han nationality

作　　者：刘柳[1] 刘晓芳 蒋超[1] 王建平[1] LIU Liu;LIU Xiaofang;JIANG Chao;WANG Jianping(Department of Neurology, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou 450052;Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052)

机构地区：[1]郑州大学第五附属医院神经内科,郑州450052 [2]郑州大学第一附属医院神经内科,郑州450052

出　　处：《郑州大学学报（医学版）》2019年第4期575-578,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目(81671165)

摘　　要：目的:研究CREB1-rs11904814、BDNF-rs6265多态性与河南汉族老年男性卒中后抑郁(PSD)的关系。方法:以319例首次发生脑卒中的河南汉族老年男性患者为研究对象,根据HAMD17项评分将患者分为PSD组(142例)、卒中对照组(177例);根据PSQI评分又将PSD患者分为伴睡眠障碍组(103例)和不伴睡眠障碍组(39例)。应用PCR-RFLP技术及基因组测序技术检测患者CREB1-rs11904814和BDNF-rs6265位点多态性。结果:PSD组BDNF-rs6265AA基因型及A等位基因频率大于卒中对照组(P<0.05),A等位基因与PSD有关联(OR=1.882,95%CI=1.344~2.635)。CREB1-rs11904814基因型及等位基因频率在PSD组和卒中对照组之间的分布差异无统计学意义(P>0.05);但CREB1-rs11904814G等位基因频率在PSD伴睡眠障碍组和不伴睡眠障碍组之间的分布存在差异(P<0.05),G等位基因与PSD伴睡眠障碍有关联(OR=2.729,95%CI=1.456~5.115)。结论:BDNF-rs6265A等位基因可能是河南汉族老年男性PSD的危险因素;CREB1-rs11904814可能与河南汉族老年男性PSD发病无关,但可能是PSD合并睡眠障碍的预测因素。Aim :To investigate whether the rs11904814 single nucleotide polymorphism(SNP) of cyclic adenosine monophosphate response element-binding protein 1(CREB1) gene and the rs6265 SNP of brain-derived neurotrophic factor(BDNF) gene were associated with post-stroke depression(PSD). Methods :A total of 319 stroke patients that were onset for the first time were adopted and classified as PSD( n =142) and control( n =177) groups according to HAMD(17 items) scores, and then the PSD group was further divided into PSD with sleep disturbance( n =103) and PSD without sleep disturbance( n =39) groups according to PSQI.PCR-RFLP and genome sequencing analyses were used to identify the genotypes of CREB1-rs11904814 and BDNF-rs6265. Results :The frequencies of AA genotype and A allele in the PSD group were significantly higher than those in the control group( P <0.05). A allele was positively associated with PSD in older males( OR =1.882, 95% CI =1.344-2.635). CREB1-rs11904814 genotypes and alleles showed no significant differences between the PSD group and the control group( P >0.05), but the frequency of G allele in the PSD with sleep disturbance group and the PSD without sleep disturbance group( P <0.05),and there was a significant correlation between G allele carriers and sleep disturbance( OR =2.729, 95% CI =1.456-5.115). Conclusion :BDNF-rs6265 A allele maybe a susceptibility factor of PSD. CREB1-rs11904814 SNP may not correlate with PSD, but may be a predictive factor for the sleep disturbance of PSD patients.

关 键 词：环磷酸腺苷反应原件结合蛋白1 脑源性神经营养因子 卒中后抑郁 睡眠障碍 

分 类 号：R741.02[医药卫生—神经病学与精神病学]