铁皮石斛叶总黄酮泡腾颗粒剂的制备与抗氧化活性研究  

Preparation and activity study of effervescent granules from flavonoids extracts of Dendrobium candidum leaves

作　　者：林雪媛 王宇轩 彭意 刘桂燕 谢俊朗 钟桂冬 罗惠玲 张泽蓉 王瑞彬 李艳萍 LIN Xueyuan;WANG Yuxuan;PENG Yi;LIU Guiyan;XIE Junlang;ZHONG Guidong;LUO Huiling;ZHANG Zerong;WANG Ruibin;LI Yanping(School of Stomatology and Medicine,Foshan University,Foshan 528000,China;Guangdong Shunde Industrial Design Institute,Foshan 528300,China)

机构地区：[1]佛山科学技术学院口腔医学院<医药工程学院>,广东佛山528000 [2]广东顺德工业设计研究院<广东顺德创新设计研究院>,广东佛山528300

出　　处：《药学研究》2019年第7期382-385,392,共5页Journal of Pharmaceutical Research

基　　金：2019年广东大学生科技创新培育专项资金项目(No.pdjh2019b0515);佛山市自筹经费类科技计划项目(No.2018AB003601);佛山科学技术学院2019年度学生学术基金重点项目

摘　　要：目的获得铁皮石斛叶黄酮提取物泡腾颗粒剂的制作工艺并测定其抗氧化能力。方法采取L9(34)正交设计法,以粒度、溶化时间为评价指标,考察乳糖的用量、柠檬酸与碳酸氢钠的配比、聚乙二醇6000(PEG6000)的用量对泡腾颗粒剂影响,以最佳处方制备泡腾颗粒剂,并采用《中国药典》2015年版方法对其进行质量评价。采用亚硝酸钠比色法测定制剂中总黄酮的含量。采用1,1-二苯基-2-三硝基苯肼(DPPH)法测定制剂的抗氧化能力。结果最佳处方为乳糖用量40%,柠檬酸与碳酸氢钠的配比1.3∶1,聚乙二醇6000用量9%。所得制剂符合《中国药典》2015年版相关标准,其总黄酮含量为(1.29±0.08)mg·g^-1,1,1-二苯基-2-三硝基苯肼清除能力的IC50为(3.77±0.10)mg·mL^-1。结论本论文将铁皮石斛叶黄酮提取物制备成吸收快、生物利用度高、便于运输携带的泡腾颗粒制剂,该制剂黄酮含量丰富,具有较好的抗氧化能力。Objective To prepare effervescent granules from Dendrobium candidum leaves and analyze its total flavonoids contents and antioxidant activity.Methods With the particle size,dissolvability time as the evaluation index,the amount of lactose,the ratio of citric acid to sodium bicarbonate and the dosage of PEG6000 which impacted response values significantly were investigated by L 9 (3 4 ) orthogonal design method to find the best of the formula.The obtained formulation was evaluated according to Chinese Pharmacopoeia(2015 edition).Its total flavonoids contents were detected by NaNO2photo colorimetric method.Its antioxidant activity was detected by DPPH method.Results The formula of effervescent granules was established as follows:the percentage of lactose and PEG6000 were 40% and 9% respectively;The ratio of citric acid to sodium bicarbonate was1.3∶1.The obtained effervescent granules met the standard of the Chinese Pharmacopoeia.The content of total flavonoids was(1.29±0.08)mg·g^-1 ,and it exerted desirable antioxidant activity with IC 50 as( 3.77 ±0.10) mg·mL^-1 .Conclusion In this experiment,the total flavonoids extract from Dendrobium candidum leaves was made into effervescent granules,which could be absorbed quickly,exerted high bioavailability and could be used conveniently.This formulation contained high number of flavonoids and had good antioxidant activity.

关 键 词：铁皮石斛叶 正交设计 黄酮类物质 抗氧化 泡腾颗粒制剂 

分 类 号：R944.27[医药卫生—药剂学]