葡萄糖醛酸木聚糖酶在枯草芽孢杆菌中的表达及发酵条件优化  

Expression of glucurono-xylanase in Bacillus subtilis and optimization of fermentation conditions

作　　者：郭亚兰 周雨朦 吴斌[1] 何冰芳[3] GUO Yalan;ZHOU Yumeng;WU Bin;HE Bingfang(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China;Nanjing Chia Tai-Tianqing Pharmaceutical Co.,Ltd.,Nanjing 222062,China;School of Pharmaceutical Sciences,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]南京正大天晴制药有限公司,江苏南京222062 [3]南京工业大学药学院,江苏南京211800

出　　处：《生物加工过程》2019年第4期379-384,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21776135)

摘　　要：为了研究葡萄糖醛酸木聚糖酶在枯草芽孢杆菌中异源表达,笔者从枯草芽孢杆菌中克隆得到带有自身信号肽的葡萄糖醛酸木聚糖酶基因,将其构建到大肠杆菌枯草芽孢杆菌穿梭质粒中,转化入枯草芽孢杆菌WB800,得到重组菌。通过发酵条件以及培养基成分优化,重组菌中葡萄糖醛酸木聚糖酶酶活达到76.0U/mL,约为优化前产酶量的5.4倍。葡萄糖醛酸木聚糖酶在枯草芽孢杆菌中实现高效异源表达,为其进一步的实际应用奠定了基础。To heterologously express glucurono-xylanase in Bacillus subtilis,we cloned the glucurono-xylanase gene with its own signal peptide from Bacillus subtilis LC-9 and constructed into E.coli-Bacillus shuttle plasmid and transformed into Bacillus subtilis WB800 to obtain the recombinant bacterium.By optimizing the culture conditions and the carbon source of the medium,the enzyme activity of the recombinant glucurono-xylanase reached 76.0 U/mL,about 5.4 times higher than that of pre-optimization.Our findings open the possibity practical application.

关 键 词：葡萄糖醛酸木聚糖酶 枯草芽孢杆菌 异源表达 穿梭质粒 木聚糖 

分 类 号：Q819[生物学—生物工程]