应用手持微型激光荧光光谱仪测定常见蔬果中的花青素  

Using Hand-Held Miniature Laser Fluorescence Spectrometer to Determine Anthocyanin in Common Fruits and Vegetables

作　　者：王世雄 李嘉宁 冉鸣[1] WANG Shi-Xiong;LI Jia-Ning;RAN Ming(College of Chemistry and Material Science, Sichuan Normal University, Chengdu 610068, China;Shanghai Shixi High School, Shanghai 200040, China)

机构地区：[1]四川师范大学化学与材料科学学院,四川成都610068 [2]上海市市西中学,上海200040

出　　处：《化学教育（中英文）》2019年第13期71-74,共4页Chinese Journal of Chemical Education

摘　　要：采用手持微型激光荧光光谱仪对几种常见蔬果中是否含有花青素进行了定性检测。实验结果表明,采用405 nm小功率激光可以有效地激发花青素荧光,并能显示出比410nm激光更显著的光谱特征,能方便、有效地在任何日常环境下实施对花青素的鉴定。所测结果与文献相吻合:桑葚、黑加仑和草莓中含花青素,而番茄、大蒜中不含。本实验操作简单,安全可靠、灵敏度高,可以作为演示实验和学生综合探究实验。Qualitative detection of the anthocyanin in several common fruits and vegetables was carried out with the hand-held miniature laser fluorescence spectrometer. The experiment indicated that the 405 nm laser could effectively excite the fluorescence of the anthocyanin with more distinct feature than that of 410nm laser. The experimental results are good consistent with literatures: mulberry, black currant and strawberry contain anthocyanin, but tomato and garlic don’t contain. This experiment was simple, safe, reliable, and with high sensitivity. It could be used as demonstration experiment and students’ comprehensive inquiry experiment.

关 键 词：花青素 激光荧光 定性检测 化学与生活 

分 类 号：O657.3[理学—分析化学] TS255.7[理学—化学]