维库溴铵对人非小细胞肺癌A549细胞株增殖和转移能力影响  

Effects of vecuronium bromide on proliferation and metastasis of human non-small cell lung cancer A549 cell line

作　　者：赵华宇[1] 刘彦辉[1] 路晓璨 刘平水[1] 董亚静[2] ZHAO Hua-yu;LIU Yan-hui;LU Xiao-can(Department of Anesthesiology,Friendship Branch,The Third Affiliated Hospital of Hebei Medical University,Shijiazhuang,Hebei Province 050051,China)

机构地区：[1]河北医科大学第三医院友谊院区麻醉科,河北石家庄050051 [2]河北省中医院麻醉科

出　　处：《中国公共卫生》2019年第7期842-846,共5页Chinese Journal of Public Health

基　　金：河北省卫生厅科研计划(20150730)

摘　　要：目的探讨维库溴铵(VB)对人非小细胞肺癌A549细胞增殖和转移能力的影响及机制。方法药物毒性实验筛选合适的剂量;细胞计数试剂盒(CCK-8)检测细胞增殖能力;Hoechst染色检测细胞凋亡情况;体外侵袭实验和划痕实验检测细胞转移能力;蛋白质印迹检测磷脂酰肌醇3激酶/蛋白激酶B(PI3K/AKT)通路相关蛋白的表达。结果与对照组比较,维库溴铵组A549细胞增殖倍数降低,细胞凋亡比率升高,侵袭细胞数目减少,划痕闭合程度显著降低;PI3K、p-PI3K和低氧诱导因子-1α(HIF-1α)的表达水平明显降低,p-AKT/AKT和p-mTOR/mTOR比值降低;PI3K激动剂740Y-P作用于A549细胞后,细胞增殖倍数升高,凋亡细胞比率降低,侵袭细胞数目增多,划痕闭合率升高;细胞中PI3K、p-PI3K和HIF-1α表达水平明显升高,p-AKT/AKT和p-mTOR/mTOR比值升高;维库溴铵与740Y-P共同作用于A549细胞,与740Y-P单独作用组比较,细胞增殖倍数降低,细胞凋亡比率升高,侵袭细胞数目减少,划痕闭合程度降低,细胞内PI3K、p-PI3K和HIF-1α表达水平降低,p-AKT/AKT和p-mTOR/mTOR比值降低。结论维库溴铵可明显抑制A549细胞增殖和转移,其机制可能与抑制PI3K/AKT通路表达有关。Objective To investigate the effect of vecuronium bromide(VB)on the proliferation and metastasis of human non-small cell lung cancer A549 cells and its possible mechanism.Methods Drug toxicity testing was used to screen appropriate dosage of VB.Cell counting kit-8(CCK-8)was used to detect cell proliferation.Hoechst staining was used to detect cell apoptosis.Transwell and wound healing were used to detect cell metastasis;Western blot was used to detect expressions of phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)pathway-related proteins.Results Compared to those of the control cells,the A549 cells treated with VB showed significantly decreased proliferation,increased apoptosis rate,increased number of invading cells,decreased wound closure rate,significantly decreased expressions of PI3K,phosphorylated PI3K(p-PI3K)and hypoxia-inducible factor-1 alpha(HIF-1α),and increased ratios of PI3K/AKT and phosphorylated mammalian target of rapamycin(p-mTOR)/mTOR;while,the A549 cells treated with 740Y-P(a PI3K agonist)exhibited increased proliferation,apoptosis rate,the number of invading cells,wound closure rate,expressions of PI3K,p-PI3K and HIF-1α,and ratios of PI3K/AKT p-mTOR/mTOR.Whereas,in comparison with the A549 cells treated only with 740Y-P,the A549 cells treated with both VB and 740Y-P demonstrated decreased proliferation,the number of invading cells,wound closure rate,expressions of PI3K,p-PI3K and HIF-1α,and ratios of PI3K/AKT p-mTOR/mTOR but increased apoptosis rate.Conclusion Vecuronium bromide can obviously inhibit the proliferation and metastasis of A549 cells possibly by inhibiting the PI3K/AKT pathway.

关 键 词：维库溴铵 非小细胞肺癌 磷脂酰肌醇-3-激酶(PI3K) 蛋白激酶B(AKT) 低氧诱导因子-1(HIF-1α) 

分 类 号：R734.2[医药卫生—肿瘤]