民猪和大白猪不同肌球蛋白重链表达差异  

Differential expression of myosin heavy chains between Min pigs and Large white pigs

作　　者：李忠秋[1,2] 刘春龙[3] 马红[1] 张冬杰[2] 孙金艳[2] 付博[2] 汪亮[2] 彭福刚[2] 郭镇华[2] 吴赛辉[2] 刘娣 LI Zhongqiu;LIU Chunlong;MA Hong;ZHANG Dongjie;SUN Jinyan;FU Bo;WANG Liang;PENG Fugang;GUO Zhenhua;WU Saihui;LIU Di(Postdoctoral Research Workstation, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;Ministry ofAgriculture Key Laboratory of Integrated Breeding and Feeding,Institute of Animal Husbandry,Heilongjiang Academy of AgriculturalSciences, Harbin 150086, China;Key Laboratory of Black Soil Ecology, Northeast Institute of Geography and Agroecology, ChineseAcademy of Sciences,Harbin 150081,China)

机构地区：[1]黑龙江省农业科学院博士后工作站,哈尔滨150086 [2]黑龙江省农业科学院畜牧研究所农业农村部种养结合重点实验室,哈尔滨150086 [3]中国科学院东北地理与农业生态研究所黑土生态重点实验室,哈尔滨150081

出　　处：《黑龙江畜牧兽医》2019年第13期49-51,57,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省农业科学院院级科研项目(2018KYJL005);现代农业产业技术体系建设专项(CARS-36);黑龙江省科研机构创新能力提升专项(YC2016D001)

摘　　要：为揭示民猪与大白猪肌纤维类型组成差异,试验应用Real-time PCR法检测了民猪和大白猪背最长肌中肌球蛋白重链(MyHC)Ⅰ、Ⅱa、Ⅱx、Ⅱb 4种亚型mRNA表达差异。结果表明:同日龄民猪和大白猪、同体重民猪和大白猪相比,民猪Ⅰ、Ⅱa、Ⅱx型MyHC mRNA相对表达量显著高于大白猪(P<0.05),而Ⅱb型MyHC mRNA相对表达量显著低于大白猪(P<0.05)。说明民猪肌肉中氧化型和中间型肌纤维含量高于大白猪,而酵解型肌纤维含量低于大白猪。In order to reveal the composition difference of muscle fiber types between Min pigs and Large white pigs,Real-time PCR method was used in this experiment for the determination of mRNA expression difference of 4 subtypes of MyHC(Ⅰ,Ⅱa,Ⅱx,Ⅱb) in longissimus dorsi of Min pigs and Large white pigs. The results showed that the contents of MyHC Ⅰ,Ⅱa,Ⅱx in Min pigs were significantly higher(P<0.05) than those in Large white pigs on the same day of age or with the same body weight,whereas that of MyHC Ⅱb in in Min pigs was significantly lower(P<0.05) than that in Large white pigs. The results suggested that the contents of fiber oxidation and middle types in Min pigs were higher than those in Large white pigs,and that of fiber glycolysis type was lower than that in Large white pigs.

关 键 词：民猪 大白猪 肌纤维类型 肌球蛋白重链(MyHC) REAL-TIME PCR 

分 类 号：S828.91[农业科学—畜牧学]