复合万古霉素后对载有BMP2的仿生磷酸钙骨替代材料成骨性能影响的研究  

The influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules

作　　者：王冬赟 罗媛[1] 彭娟红[1] WANG Dongyun;LUO Yuan;PENG Juanhong(Department of Stomatology,Beijing University Shenzhen Hospital,Shenzhen 518035,Guangdong Province,China)

机构地区：[1]北京大学深圳医院口腔医学中心

出　　处：《中国口腔种植学杂志》2019年第2期51-54,共4页Chinese Journal of Oral Implantology

基　　金：国家自然科学基金青年基金项目(31800808)

摘　　要：目的:探讨复合万古霉素后对载有BMP2的仿生磷酸钙骨替代材料(BMP2-coprecipated biomimetic calcium phosphate granules,BioCaP-BMP2)成骨性能的影响。方法:在BioCaP-BMP2上接种BMSCs,观察不同浓度(0,1, 10,30μg/ml)万古霉素对细胞生物学行为的影响。采用CCK8法在培养1、4、7天时检测BMSCs的增殖;第4、7天检测总蛋白浓度与碱性磷酸酶活性及骨钙素的表达。结果:相比对照组,1μg/ml、10μg/ml组中BMSCs表现出与对照组相当的增殖和成骨分化能力,而30μg/ml组万古霉素显示对BMSCs的抑制作用。结论:治疗浓度的万古霉素不会影响BioCaP-BMP2的成骨效果,构建复合万古霉素的BioCaP-BMP2的颗粒有望成为治疗感染性骨缺损的理想材料。Objective: To investigate the influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules. Methods: After being seeded on BioCaP-BMP2 granules,BMSCs were treated with vancomycin at concentration of 0,1, 10, 30μg/ml. Cell proliferation was detected at 1, 4, 7 days by CCk8 assay. Total protein, alkaline phosphatase activity and osteocalcin expression were examined at 4 and 7 days. Results: Compared with control group, vancomycin at concentration of 1 and 10μg/ml did not show negative effects on the biological behavior of BMSCs. On the contrary, high concentration of vancomycin was found to inhibit the proliferation and osteogenic differentiation of BMSCs. Conclusion: Therapeutic concentration of vancomycin will not negatively influence the osteogenic efficacy of BioCaP-BMP2, which means BioCaP-BMP2 granules carrying vancomycin could be a promising material to treat infectious bone defects.

关 键 词：骨髓间充质干细胞 万古霉素 骨形态发生蛋白-2 成骨分化 

分 类 号：R783.1[医药卫生—口腔医学]