RanBPM调控IFN-λR1-STAT5通路分子机制研究  

Molecular Mechanism of RanBPM Regulating IFN-λR1-STAT5 Signaling Pathway

作　　者：张俊文[1,2] 赵乔佳杰 杨霞 马雯 黄秉仁 刘福生 Zhang Junwen;Zhao Qiaojiajie;Yang Xia(Beijing Neurosurgical Institute,Beijing Tiantan Hospital Affiliated to Capital Medical University,Beijing 100070,China)

机构地区：[1]北京市神经外科研究所脑肿瘤研究中心、首都医科大学附属北京天坛医院,100070 [2]中国医学科学院基础医学研究所/北京协和医学院基础学院,100005

出　　处：《医学研究杂志》2019年第8期31-36,共6页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81672478);北京市自然科学基金资助项目(7151002);生物医用材料北京实验室基金资助项目(京教函[2013]133号)

摘　　要：目的研究RanBPM调控IFN-λR1-STAT信号通路的分子机制。方法利用免疫共沉淀和双荧光素酶报告基因等方法检测IFN-λR1中与STAT5A结合的位点,RanBPM与STAT5A的相互作用和相互作用的结构域,以及相互作用后对STAT5A报告基因活性的影响。结果IFN-λR1中含有两个与STAT5A结合的位点;STAT5A能与RanBPM相互作用,RanBPM的氨基酸和羧基端有与STAT5A相互作用的位点;RanBPM增强了STAT5A双荧光素酶报告基因活性。结论RanBPM能分别与IFN-λR1和STAT5A相互作用,参与调控IFN-λR1-STAT5通路。Objective To investigate the molecular mechnism of RanBPM regulating IFN-λR1-STAT5A signalling pathway.Methods Cell culture,cell transfection,immunoprecipitation and dual-luciferase reporter assay were peformed to check the interaction of IFN-λR1 and TRAF6,RanBPM and STAT5A,the interacting domain and the consequence of STAT5A reporter activity.Results IFN-λR1 harbored two STAT5A-binding sequence.RanBPM can interact with STAT5A and the interaction motif was located in amino terminus and the carboxy terminus,respectively.After interaction,RanBPM enhanced the STAT5A dual-luciferase reporter gene activity.Conclusion RanBPM interacts with IFN-λR1 and STAT5A,respectively,and is involved in the regulation of the IFN-λR1-STAT5 pathway.

关 键 词：RANBPM 相互作用 IFN-λR1 STAT5A 

分 类 号：R73[医药卫生—肿瘤]