Pre-incubated with BSA-complexed free fatty acids alters ER stress/autophagic gene expression by carboxylated multi-walled carbon nanotube exposure in THP-1 macrophages  

作　　者：Qianyu Yang Maolin Wang Yongbing Sun Shengming Peng Yanhuai Ding Yi Cao 

机构地区：[1]Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, China [2]National Engineering Research Center for Solid Preparation Technology of Chinese Medicines, Jiangxi University of Traditional Chinese Medicines, Nanchang 330006, China

出　　处：《Chinese Chemical Letters》2019年第6期1224-1228,共5页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (No. 21707114);Scientific Research Foundation of Hunan Provincial Education Department (No. 17A205)

摘　　要：Recently we found that multi-walled carbon nanotube (MWCNT) exposure alters the mRNA levels of endoplasmic reticulum (ER) stress/autophagic genes, but the impact of biological molecules on this response is unclear. Herein, we compared the different actions of carboxylated MWCNTs (c-MWCNTs) pre-incubated with bovine serum albumin (BSA) or BSA-complexed free fatty acid (denoted as FFA) on macrophages derived from THP-1 monocytes (denoted as THP-1 macrophages). C-MWCNTs exhibited increased diameter and hydrodynamic size as well as decreased absolute zeta potential value after pre-incubation with BSA or FFA, which suggested a coating effect. Cytotoxicity or oxidative stress were not significantly induced after exposure to BSA-or FFA-coated c-MWCNTs. BSA-pre-incubated c-MWCNTs significantly enhanced the expression of the ER stress gene, DDIT3 and the autophagic genes, ATG5, BECN1, and PLIN2, but the mRNA levels of these genes was significantly decreased by FFA-pre-incubated c-MWCNTs. FFA-pre-incubated c-MWCNTs induced significantly higher lipid accumulation and interleukin-6 (IL-6) protein level compared with BSA-pre-incubated c-MWCNTs, which suggested that FFA-pre-incubated c-MWCNTs may more effectively induce the formation of macrophage foam cells. Collectively, our data indicated that pre-incubation with FFA may influence c-MWCNT-induced ER stress/autophagic gene expression and foam cell formation in THP-1 macrophages.Recently we found that multi-walled carbon nanotube(MWCNT)exposure alters the mRNA levels of endoplasmic reticulum(ER)stress/autophagic genes,but the impact of biological molecules on this response is unclear.Herein,we compared the different actions of carboxylated MWCNTs(c-MWCNTs)pre-incubated with bovine serum albumin(BSA)or BSA-complexed free fatty acid(denoted as FFA)on macrophages derived from THP-1 monocytes(denoted as THP-1 macrophages).C-MWCNTs exhibited increased diameter and hydrodynamic size as well as decreased absolute zeta potential value after preincubation with BSA or FFA,which suggested a coating effect.Cytotoxicity or oxidative stress were not significantly induced after exposure to BSA-or FFA-coated c-MWCNTs.BSA-pre-incubated c-MWCNTs significantly enhanced the expression of the ER stress gene,DDIT3 and the autophagic genes,ATG5,BECN1,and PLIN2,but the mRNA levels of these genes was significantly decreased by FFA-pre-incubated c-MWCNTs.FFA-pre-incubated c-MWCNTs induced significantly higher lipid accumulation and interleukin-6(IL-6)protein level compared with BSA-p re-incubated c-MWCNTs,which suggested that FFA-p re-incubated c-MWCNTs may more effectively induce the formation of macrophage foam cells.Collectively,our data indicated that pre-incubation with FFA may influence c-MWCNT-induced ER stress/autophagic gene expression and foam cell formation in THP-1 macrophages.

关 键 词：Multi-walled carbon nanotubes (MWCNTs) THP-1 MACROPHAGES Endoplasmic reticulum (ER) stress Autophagy Foam cells 

分 类 号：O6[理学—化学]