双水相萃取法测定灯盏花及提取物中灯盏花乙素含量  

Determination of Scutellarin in Breviscapus and Its Extract by Aqueous Two-phase System

作　　者：肖丹[1] 胡昕 杨万林 李晚谊[1] 李智敏[1] 吴丽华[1] 张庭[1] 杨德志 杨亚玲[3] 王家金[1] XIAO Dan;HU Xin;YANG Wan-lin;LI Wan-yi;LI Zhi-min;WU Li-hua;ZHANG Ting;YANG De-zhi;YANG Ya-ling;WANG Jia-jin(Institute of Medicinal Plant, Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650205 , China;Institute of Agro-products Processing, Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650223 , China;Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Kunming 650500, China)

机构地区：[1]云南省农业科学院药用植物研究所,云南昆明650205 [2]云南省农业科学院农产品加工研究所,云南昆明650223 [3]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《西南农业学报》2019年第7期1555-1559,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技厅重大科技专项(农业)云南道地药材产地标准化初加工和贮运技术研究与示范(2017AB004)

摘　　要：【目的】研究建立由乙醇和(NH_4)_2SO_4形成的双水相体系萃取灯盏花中灯盏花乙素。【方法】用HPLC法对灯盏花乙素含量进行测定,并对影响双水相体系的成相和灯盏花乙素萃取率的条件进行优化。【结果】灯盏花粗提液经过双水相体系萃取之后,能有效减低大分子物质和极性分子的干扰。乙醇体积为1.5 mL、(NH_4)_2SO_4质量分数为25%、pH为6.0时,双水相体系对灯盏花乙素的萃取率达到94.2%。【结论】对灯盏花样品进行的检测和加标回收实验表明,该方法准确性较高,结果可靠,操作简单,绿色无污染。【Objective】 A new method for detecting scutellarin in breviscapus by Aqueous Two-phase System(ATPS) formed by ethanol and(NH4)2SO4 was investigated. 【Method】 The conditions affecting the phase formation of ATPS and the extraction efficiency of the scutellarin were optimized. 【Result】 After the breviscapus crude extract by ATPS, the interference of macromolecular substances and polar molecules could be effectively reduced. The best ATPS was composed of 1.5 mL ethanol and 25 %(NH4)2SO4 and pH 6.0. And the extraction efficiency of scutellarin reached about 94.2 %. 【Conclusion】 The detection and recovery experiments of breviscapine samples showed that the method was accurate, reliable, easy to operate and green and pollution-free.

关 键 词：双水相 灯盏花 绿色环保 灯盏花乙素 

分 类 号：R286.02[医药卫生—中药学]