Spectroscopic and molecular docking studies on the binding mechanism of Mobic and lipase  

作　　者：Baosheng Liu Xu Cheng Hongcai Zhang 

机构地区：[1]College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, National Chemistry Experimental Teaching Demonstration Center, Hebei University, Baoding 071002, China

出　　处：《Journal of Pharmaceutical and Biopharmaceutical Research》2019年第2期53-60,共8页制药与生物制药研究杂志（英文）

摘　　要：Under simulated physiological conditions (pH=7.40), the interaction between non-steroidal anti-inflammatory drug Mobic and lipase was studied by fluorescence spectra, ultraviolet absorption spectra, circular dichroism spectra and computer simulation technique. The experimental results showed that Mobic could quench the fluorescence of lipase by static quenching, and the binding site number is about 1. According to F¨orster’s theory of non-radiation energy transfer, the binding distance between Mobic and lipase was obtained, r<7 nm, which indicated that there was non-radiation energy transfer in the system. The thermodynamic parameters were obtained from van’t Hoff equation, Gibbs free energy -G<0, indicating that the reaction between them was spontaneous,-H<0,-S>0, indicating that hydrophobic force played a major role in the formation of Mobic and lipase complex. The results of synchronous fluorescence spectra, UV spectra and circular dichroism spectra showed that Mobic changed the conformation of lipase. The molecular docking results showed that the binding position of Mobic was close to the active center, indicating that Mobic could change the microenvironment of amino acid residues at the active center of lipase catalysis. The results of docking showed that there was hydrogen bond between Mobic and lipase, so the interaction between Mobic and lipase was driven by hydrophobic interaction and hydrogen bond.

关 键 词：SPECTROSCOPY Mobic LIPASE CONFORMATION MOLECULAR DOCKING interaction 

分 类 号：R[医药卫生]