香菇菌丝体对四种抗生素的敏感性  

Sensitivity of Mycelia of Lentinula edodes Against Four Antibiotics

作　　者：姜宁[1] 章炉军[1] 谭琦[1] 尚晓冬[1] 董浩然 JIANG Ning;ZHANG Lu-jun;TAN Qi;SHANG Xiao-dong;DONG Hao-ran(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所国家食用菌工程技术研究中心

出　　处：《黑龙江农业科学》2019年第8期119-123,共5页Heilongjiang Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项资金(CARS-20);上海市食用菌产业技术体系建设专项资金资助(沪农科产字(2019)第9号)

摘　　要：为探究4种常用抗生素对香菇菌丝体生长的影响,筛选适用于香菇遗传转化的抗生素。本研究以香菇[Lentinula edodes(Berk.)Pegler]沪香F2双核菌丝以及其原生质体单核菌丝为材料,通过测试两者在含有不同种类、不同浓度下抗生素培养皿中的生长速率以及抑制率,探究香菇单双核菌丝对氨苄青霉素、卡那霉素、头孢霉素、潮霉素4种抗生素的敏感性。结果表明:氨苄青霉素、卡那霉素、头孢霉素对香菇单双核菌丝生长以及菌落形态基本没有影响,潮霉素对香菇单双核菌丝生长有强烈的抑制作用,12mg·L^-1的潮霉素能够完全抑制香菇双核菌丝的生长,14mg·L^-1的潮霉素能够完全抑制香菇单核菌丝的生长。卡那霉素不能作为香菇遗传转化的筛选抗生素使用;潮霉素可以用作香菇遗传转化的筛选抗生素;氨苄青霉素和头孢霉素均可以用于对农杆菌的抑制。In order to investigate the effects of four antibiotics on the mycelium growth of lentinus edodes,screen out suitable antibiotics for genetic transformation of lentinus edodes,we used the mycelium of Lentinula edodes(Berk.)Pegler-huxiang F2 as material,growth rates and inhibition rates were measured in petri dishes containing different types and concentration,the research aimed to investigate that resistance of mycelia of Lentinula edodes against four kind antibiotics,including ampicillin,kanamycin,cefotaxime and hygromycin.The results showed that ampicillin,kanamycin,cefotaximehad no effect on mycelia growth and colony morphology of Lentinula edodes,hygromycin had a strong inhibitory effect on the growth of mushroom mycelium,hygromycin at 12 mg·L^-1 could completely inhibit the growth of Lentinula edodes dikaryon mycelia,hygromycin at14 mg·L^-1 could completely inhibit the growth of Lentinula edodes monokaryon mycelia.Kanamycin cannot be used as a screening antibiotic for genetic transformation of Lentinula edodes.Hydramycin can be used as a screening antibiotic for genetic transformation of Lentinula edodes.Ampicillin and cefotaxime could be used to inhibit Agrobacterium.

关 键 词：香菇菌丝 抗生素 生长速率 抑制率 

分 类 号：S646.12[农业科学—蔬菜学]