表没食子儿茶素没食子酸酯对脊髓损伤后小鼠神经功能的恢复作用及机制研究  

Protective effects of epigallocatechin gallate on neural function in mice with spinal cord injury and its mechanism

作　　者：吴凯 封志云[1] 胡小坚 WU Kai;FENG Zhiyun;HU Xiaojian(Department of Orthopedics,the First Affiliated Hospital, Zhejiang University, Hangzhou 310003,China)

机构地区：[1]浙江大学医学院附属第一医院骨科

出　　处：《浙江医学》2019年第14期1464-1467,1476,共5页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划项目(2017KY053)

摘　　要：目的探讨表没食子儿茶素没食子酸酯(EGCG)对脊髓损伤(SCI)后小鼠神经功能的恢复作用及相关机制。方法采用挤压型方法制作小鼠急性SCI动物模型,采用随机数字表法将造模成功的20只小鼠分为EGCG组和SCI组,每组10只。另10只未造模的小鼠仅行椎板切除,作为假手术组。EGCG组小鼠予EGCG(10ml/kg)腹腔注射;假手术组和SCI组小鼠予0.9%氯化钠注射液(10ml/kg)腹腔注射;均1次/d,连续7d。术后第1、3、5、7天进行小鼠BMS评分;取脊髓组织HE染色观察细胞形态变化,免疫组化法检测脊髓组织Cleaved Caspase-3表达水平,Western blot法检测Cleaved Caspase-3、B细胞淋巴瘤因子2(Bcl-2)、Bcl-2相关X蛋白(Bax)蛋白表达水平。结果与假手术组比较,SCI组和EGCG组小鼠BMS评分均降低,脊髓组织Cleaved Caspase-3、Bax蛋白表达水平均升高,Bcl-2蛋白表达水平均降低,差异均有统计学意义(均P<0.05);与SCI组比较,EGCG组小鼠BMS评分升高,脊髓组织Cleaved Caspase-3、Bax蛋白表达水平均降低,Bcl-2蛋白表达水平升高,差异均有统计学意义(均P<0.05)。结论 EGCG可明显促进SCI后小鼠神经功能的恢复,其发挥神经保护作用的机制可能与其抑制神经细胞凋亡密切相关。Objective To investigate the effects of epigallocatechin gallate(EGCG) on neural function in mice with spinal cord injury(SCI), and to explore its mechanism. Methods SCI model was induced by clamping the spinal cord in mice. Twenty SCI mice were randomly divided into EGCG group and SCI group with 10 in each group. Ten mice in sham group received the sham procedure without spinal cord injury. The mice in EGCG group were injected intraperitoneally with EGCG(10 ml/kg) q. d for 7 d;the mice in sham group and SCI group received same volume of saline q. d for 7 d. At 1, 3, 5 and 7 d after SCI, the neural function was assessed with Basso Mouse Scale( BMS). The morphological changes of spinal cord were observed by light microscopy with HE staining. The expression of Cleaved Caspase-3, Bcl-2 and Bax was observed by immunochemistry and Western blot. Results Compared with the mice in sham group, the BMS scales in SCI group and EGCG group decreased, the expression of Caspase-3 and Bax increased, and the expression of Bcl-2 decreased(all P<0.05). Compared with the mice in the SCI group, the BMS scales in EGCG group increased, the expression of Caspase-3 and Bax decreased, and the expression of Bcl-2 increased(all P<0.05). Conclusion EGCG can promote the recovery of neural function in mice with experimental spinal cord injury, which may be related to its anti-apoptotic effects.

关 键 词：脊髓损伤 表没食子儿茶素没食子酸酯 凋亡 小鼠 

分 类 号：R651.2[医药卫生—外科学] R-332[医药卫生—临床医学]