猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用  

Effect of Porcine Antibacterial Peptide against Avian Pathogenic Bacteria

作　　者：刘小龙 赖隆永 谭礼宁 刘友霖 徐磊[2,3] 吴昌标 杨守深 杨慧[2] 余勋信[2] 程龙飞[6] 黄瑜[6] LIU Xiao-long;LAI Long-yong;TAN Li-ning;LIU You-lin;XU Lei;WU Chang-biao;YANG Shou-shen;YANG Hui;YU Xun-xin;CHENG Long-fei;HUANG Yu(Present (Fuzhou) Biotech Co.,Ltd., Fuzhou, Fujian 350500,China;Fujian Vocational College of Agriculture,Fuzhou, Fujian 350303, China;Nanjing Tianbang Bio-industry Company Limited, Nanjing, Jiangsu 211102,China;Fujian Vocational College of Agriculture, Fuzhou, Fujian350007, China;College of Life Sciences,Longyan University, Longyan, Fujian 364012, China;Instituteof Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian350013, China)

机构地区：[1]派生特(福州)生物科技有限公司,福建福州350500 [2]福建农业职业技术学院,福建福州350303 [3]南京天邦生物科技有限公司,江苏南京211102 [4]福建生物工程职业技术学院,福建福州350007 [5]龙岩学院生命科学学院,福建龙岩364012 [6]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《福建农业学报》2019年第5期575-580,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目(2019N0025、2017N0009);福建省科技计划项目(2016S0002);福建省中青年教师教育科研项目(JZ180531);福建省禽病防治重点实验室开放基金课题(KF201902);2019年度江苏省博士后科研资助计划[苏人社发(2019)104号];福州市科技计划项目(2019-G-34);福建省动物保健与食品安全应用技术协同创新中心项目[闽教科(2017)49号];福建省畜禽疫病防控技术重大研发平台项目(2014N2003)

摘　　要：【目的】明确猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用,为新型抗菌药物的研发和猪源抗菌肽的生产应用提供科学依据。【方法】以猪源抗菌肽为材料,采用液体培养基抑制测定法,结合体内外试验,分别测定猪源抗菌肽对禽源沙门氏菌和禽源大肠杆菌的抗菌活力值,研究其体内外抑菌作用。【结果】对不同浓度的抗菌肽抗菌活力值的测定结果表明,与0.5、0.2和0.1mg·mL-1的猪源抗菌肽相比较,1.0mg·mL-1的抗菌肽对含有106CFU·mL-1的菌液抑菌效果最好;与阿莫西林、红霉素、四环素等15种抗生素相比较,猪源抗菌肽对禽源沙门氏菌FJS13株和禽源大肠杆菌FJE61株的抑菌圈最大,其抗菌效果最好;抗菌肽在雏鸡体内的抑菌试验表明,攻毒鸡皮下注射猪源抗菌肽有利于提高雏鸡的存活率,在雏鸡体内也具有一定的抑菌作用。【结论】猪源抗菌肽可用于抑制禽源沙门氏菌和大肠杆菌。【Objective】 Activity of porcine antibacterial peptides (PAP) against avian Salmonella and Escherichia coli was determined for drug development.【Method】 Inhibitive activity of PAP on avian pathogens was studied in vitro on an liquid medium and by an in vivo test.【Results】 PAP at a concentration of1.0 mg·mL -1 showed a higher bacteriostatic effect on the solution contaning10 6 CFU·mL -1 of the pathogens than at 0.5, 0.2 or 0.1 mg·mL -1 .Compared with15 antibiotics, such as amoxicillin, erythromycin, tetracycline, etc., PAP induced the largest inhibition zone on the growth of avian Salmonella FJS13 and E.coli FJE61.In a challenging test on chicken, the PAP administration improved the survival rate of the subcutaneous injected birds.【Conclusion】 PAP could be used as a bioagent to combat the infection on chickens by avian Salmonella and E.coli .

关 键 词：猪源抗菌肽 抗菌活力值 抑制作用 沙门氏菌 大肠杆菌 雏鸡 

分 类 号：S852.6[农业科学—基础兽医学]