血管内皮生长因子表达与丹参预防肝小静脉闭塞病机制的研究  

The expression of VEGF in preventive mechanism of Salvia miltiorrhiza on hepatic veno-occlusive disease

作　　者：杨丽[1] 王向阳[1] 徐晓平[1] 陈哲[2] 朱洪怡[3] 霍继荣[3] YANG Li;WANG Xiangyang;XU Xiaoping;CHEN Zhe;ZHU Hongyi;HUO Jirong(Department of Gastroenterology,Hunan Provincial People’s Hospital,Changsha 410005;Department of Geriatrics,Changsha CentralHospital;Department of Gastroenterology,the Second Xiangya Hospital of Central South University,China)

机构地区：[1]湖南省人民医院消化内科,湖南长沙410005 [2]长沙市中心医院老年科 [3]中南大学湘雅二医院消化内科

出　　处：《胃肠病学和肝病学杂志》2019年第7期731-734,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的利用不同剂量丹参干预土三七诱导小鼠肝小静脉闭塞病(HVOD)模型,检测血管内皮生长因子(VEGF)表达,探讨丹参预防HVOD发生的作用机制。方法将100只小鼠随机分为4组,分别予磷酸缓冲液(PBS)、土三七、土三七+100mg/kg丹参、土三七+200mg/kg丹参灌胃,连续30d后处死小鼠。通过修改后Deleve评分标准评价小鼠HVOD成模情况;免疫组化检测VEGF蛋白表达水平;RT-PCR检测VEGFmRNA水平。结果丹参干预组与土三七模型组比较,VEGF蛋白及mRNA表达水平显著降低(P<0.05),200mg/kg丹参组的VEGF表达水平较100mg/kg丹参组下降更明显(P<0.05)。结论不同剂量丹参可减少土三七诱导小鼠HVOD的发生,抑制VEGF的表达可能是丹参预防HVOD发生的重要作用机制之一。Objective To investigate the mechanism of Salvia miltiorrhiza in preventing mice hepatic veno-occlusive disease (HVOD) induced by gynura segetum,VEGF expression was detected and different concentrations of Salvia miltiorrhiza was used to prevent the HVOD. Methods One hundred female Kunming mice were randomly classified into four groups.The control group was treated with PBS,model group was induced by gynura segetum,gynura segetum groups preventively treated with Salvia miltiorrhiza at two dosages (gynura segetum+Salvia miltiorrhiza,100 mg/kg,200 mg/kg).The mice were sacrificed on the 30st day.The modified Deleve scoring standard was used to evaluate the modeling of mice HVOD.The expression of VEGF was detected by immunohistochemistry and RT-PCR. Results Compared with gynura segetum group,the Salvia miltiorrhiza groups had significant decreased in the level of VEGF ( P <0.05). Conclusion Salvia miltiorrhiza with different concentrations has therapeutic effect on HVOD induced by gynura segetum,and inhibition expression of VEGF may be one of the important mechanisms of Salvia miltiorrhiza in preventing HVOD.

关 键 词：肝小静脉闭塞病 丹参 血管内皮生长因子 

分 类 号：R575.29[医药卫生—消化系统]