分蘖洋葱鳞茎总黄酮提取工艺及抗氧化活性  

Extraction Process and Antioxidant Activity of Total Flavonoids From the Bulbs of Multiplier Onion(Allium cepa L. var. Aggregatum G. Don)

作　　者：黄云彤 娄虎[2] 刘学 崔成日 徐启江[2] HUANG Yuntong;LOU Hu;LIU Xue;CUI Chengri;XU Qijiang(Heilongjiang Nursing College,Harbin,Heilongjiang 150086;College of Life Sciences,Northeast Forestry University,Harbin,Heilongjiang 150040;Harbin Allium Agricultural Science and Technology Co. Ltd.,Harbin,Heilongjiang 150300)

机构地区：[1]黑龙江护理高等专科学校,黑龙江哈尔滨150086 [2]东北林业大学生命学院,黑龙江哈尔滨150040 [3]哈尔滨艾利姆农业科技有限公司,黑龙江哈尔滨150300

出　　处：《北方园艺》2019年第14期106-116,共11页Northern Horticulture

基　　金：黑龙江省科学基金资助项目(C2018002);哈尔滨市应用技术研究与开发项目-中小企业创新基金资助项目(2016FF6GJ009)

摘　　要：以分蘖洋葱‘M-3’鳞茎为试材,用超声波辅助提取黄酮类化合物,在单因素试验基础上,采用Box-Behnken响应面法优化分蘖洋葱总黄酮提取工艺,用DPPH法对总黄酮化合物抗氧化活性进行评价。结果表明:最佳提取工艺参数为料液比1∶45 g·mL^-1、乙醇体积分数60%、提取时间40 min、提取温度48℃、超声波功率310 W。在该条件下,分蘖洋葱总黄酮的提取率为3.584%,与模型预测值3.622%相近。分蘖洋葱总黄酮提取物对DPPH·具有一定的清除作用,IC50值为0.179 mg·mL^-1。优选的超声波提取工艺提高了分蘖洋葱总黄酮提取率,具有较强的抗氧化活性。Taking the bulbs of’M-3’multiplier onion(Allium cepaL.var.Aggregatum G.Don)as test material,on the basis of single factor experiment,the optimum ultrasound-assisted extraction process of total flavonoids from bulbs was analyzed by using Box-Behnken design(BBD)with response surface methodology.The antioxidant activity of total flavonoids was evaluated by DPPH method.The results showed that the optimal extraction conditions were determined as follows:1∶40 g·mL^-1 of solidliquid ratio,60%of ethanol volume fraction,50℃of extraction temperature,300 W of ultrasonic power,30 minutes of extraction time.Under these conditions,the exaction rate of total flavonoids from the bulbs of multiplier onion was 3.586%,which was closed to the model predicted value of 3.622%.The antioxidant tests showed the total flavonoids had a scavenging effect on DPPH·.The IC50 value was 0.179 mg·mL^-1 which indicated that the flavonoids from multiplier onion bulbs has strong antioxidant activity.The optimal ultrasonic-assisted extraction process improved the extraction rate of total flavonoids from the bulbs of multiplier onion,and the extracts by this process also exhibited a better antioxidant activity.

关 键 词：分蘖洋葱 总黄酮 超声提取 响应面法 抗氧化活性 

分 类 号：S633.2[农业科学—蔬菜学]