RUNX3和p53在结直肠锯齿状病变及结直肠癌组织中的表达及意义  

Expression and significance of RUNX3 and p53 in colorectal serrated lesions and colorectal cancer

作　　者：赵巍[1] 李琳[1] 张肖洁[1] 何英[1] 蒋萍[1] 龙培滨[1] 柳平平 王丹丹 ZHAO Wei;LI Lin;ZHANG Xiao-jie;HE Ying;JIANG Ping;Long Pei-bin;LIU Ping-ping;Wang Dan-dan(Department of Gastroenterology,Harbin First Hospital,Heilongjiang Province,Harbin 150010,China)

机构地区：[1]哈尔滨市第一医院消化科

出　　处：《中国当代医药》2019年第20期40-42,共3页China Modern Medicine

基　　金：黑龙江省卫生计生委科研课题(2014-002)

摘　　要：目的探讨RUNX3和p53在结直肠锯齿状病变及结直肠癌组织中的表达及意义。方法选择2012年1月~2018年1月我院病理科确诊的262例结直肠锯齿状病变,其中增生性息肉(HP)87例,广基锯齿状息肉/腺瘤(SSA/P)96例,传统锯齿状腺瘤(TSA)79例;另选取60例正常肠黏膜标本以及60例结直肠癌(CRC)标本。通过免疫组化染色对各标本中RUNX3和p53的表达进行分析和评价。结果RUNX3阳性表达率在正常肠黏膜、HP、SSA/P、TSA、CRC中逐渐降低,在SSA/P、TSA及CRC中明显降低;SSA/P、TSA、CRC与正常肠黏膜及HP的RUNX3阳性率比较,差异有统计学意义(P<0.05)。p53阳性表达率在正常肠黏膜、HP、SSA/P、TSA、CRC中逐渐升高,在SSA/P、TSA及CRC中明显升高;SSA/P、TSA、CRC与正常肠黏膜及HP的p53阳性率比较,差异有统计学意义(P<0.05)。结论RUNX3和p53在锯齿状病变到CRC的发生过程中起作用,两者可能是促进锯齿状腺瘤恶变的危险因素,对锯齿状腺瘤的患者预测CRC的发生具有一定的临床价值。Objective To investigate the expression and significance of RUNX3 and p53 in colorectal serrated lesions and colorectal cancer.Methods A total of 262 specimens of colorectal serrated lesions diagnosed in the pathology department of our hospital from January 2012 to January 2018 were selected,including 87 cases of hyperplastic polyps (HP),96 cases of sessile serrated adenoma/polyp (SSA/P),and 79 cases of traditional serrated adenoma (TSA).Another 60 normal intestinal mucosa and 60 colorectal cancer (CRC) specimens were selected.The expression of RUNX3 and p53 in each specimen was analyzed and evaluated by immunohistochemical staining.Results The positive expression rate of RUNX3 gradually decreased in normal intestinal mucosa,HP,SSA/P,TSA,CRC,and significantly decreased in SSA/P,TSA and CRC.The positive rate of RUNX3 between SSA/P,TSA,CRC and normal intestinal mucosa,HP was statistically significant (P<0.05).The positive expression rate of p53 increased gradually in normal intestinal mucosa,HP,SSA/P,TSA,CRC,and increased significantly in SSA/P,TSA and CRC.The positive rate of p53 between SSA/P,TSA,CRC and normal intestinal mucosa,HP was statistically significant (P<0.05).Conclusion RUNX3 and p53 play the roles in the occurrence of serrated lesions to CRC.They may be the risk factors of the progress of serrated adenoma to adenocarcinoma.The detection of RUNX3 and p53 in serrated adenoma patients can help to predict the occurrence of CRC.

关 键 词：RUNX3 P53 结直肠锯齿状病变 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]