急性pH和碳酸盐碱度对缢蛏存活率、Na^+/K^+-ATPase活性及血淋巴吞噬能力的影响  

Effects of pH and carbonate alkalinity on survival rate, Na^+/K^+-ATPase activity and phagocytic ability of the razor clam(Sinonovacula constricta)

作　　者：叶博 程之扬 彭茂潇 牛东红[1,2] 刘晓军 蓝天一[1] 无 YE Bo;CHENG Zhiyang;PENG Maoxiao;NIU Donghong;LIU Xiaojun;LAN Tianyi(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Centre for Aquatic Animal Genetics and Breeding,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [2]上海海洋大学水产动物遗传育种中心上海市协同创新中心,上海201306 [3]上海海洋大学上海水产养殖工程技术研究中心,上海201306

出　　处：《水产学报》2019年第8期1723-1732,共10页Journal of Fisheries of China

基　　金：国家自然科学基金(31472278);国家“八六三”高技术研究发展计划(2012AA10A400)~~

摘　　要：采用静态毒理学实验方法,分析了2种规格缢蛏(小规格SSc和大规格LSc)在pH和碳酸盐碱度(CA)急性胁迫条件下的存活率,Na^+/K^+-ATPase(NKA)活性以及血淋巴的吞噬能力。结果显示,当CA浓度为2.5 mmol/L、pH值为7.5~9.5时,2种规格缢蛏的存活率均接近100%;当pH值大于9.5时,2种规格缢蛏的存活率均显著下降。当CA浓度为0~44.58mmol/L、pH值为9.0~10.0时,随着CA浓度的上升,缢蛏的存活率明显下降;在pH值为9.5条件下,LSc的鳃组织NKA活性,随着CA浓度的上升而升高,LSc血淋巴的吞噬能力随着CA浓度的上升而下降。由此可见,缢蛏在高pH或高CA下表现出较强的耐受性,但高pH和高CA协同胁迫下对缢蛏的存活率具有较大的影响,研究结果为进一步探索缢蛏在盐碱地的养殖提供了一定的理论参考。In this study,a static toxicology experiment was conducted to analyze the survival rate,Na^+/K^+-ATPase(NKA)activity and phagocytic ability of the hemolymph of Sinonovacula constricta of two different sizes(smallsize SSc and large-size LSc)under the acute stress of high pH and carbonate alkalinity(CA).The results showed that when the CA concentration was 2.5 mmol/L and the pH was 7.5-9.5,the survival rates of the two sizes were close to 100%.When the pH value was greater than 9.5,the survival rates of the two sizes were significantly decreased.When the concentration of CA was 0-44.58 mmol/L and the pH value was 9.0-10.0,the survival rate of clams decreased with the increase of CA concentration.Under the condition of pH 9.5,the NKA activity of LSc gill tissue increased with the increase of CA concentration,and the phagocytic capacity of LSc haemolymph decreased with the increase of CA concentration.It can be seen that S.constricta showed strong tolerance to high pH or CA,but high pH and CA had a great effect on the survival rate of S.constricta.The research results provide a theoretical reference for further exploration of breeding of S.constricta in saline-alkali soil.

关 键 词：缢蛏 PH 碳酸盐碱度 存活率 Na^+/K^+-ATPase活性 吞噬能力 内陆盐碱水 

分 类 号：S917.4[农业科学—水产科学]