链霉亲和素及其亲和系统的蛋白质进化  

Protein Evolution of Streptavidin and Its Affinity System

作　　者：张檬 罗棉兴 陈国[1] ZHANG Meng;LUO Mian-Xing;CHEN Guo(School of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021 , Fujian, China)

机构地区：[1]华侨大学生物工程与生物技术系

出　　处：《中国生物化学与分子生物学报》2019年第7期722-729,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.21576108);福建省新世纪优秀人才项目资助;华侨大学研究生科研创新基金资助项目~~

摘　　要：链霉亲和素/生物素(Streptavidin/Biotin)体系作为目前已知的最高亲和力作用体系,已在生物学研究中获得广泛应用。本文针对Streptavidin/Biotin和Strep-Tactin/Strep-tag两个相关系统的演化,分别从链霉亲和素蛋白的结构改造、亲和肽标签优化等方面进行了较为详细的归纳。通过对链霉亲和素蛋白各种突变体的优缺点的比较,有助于实际应用中选择合适的Streptavidin突变体。本文通过对链霉亲和素蛋白质进化的综述,可帮助更准确地理解市场上各种链霉亲和素蛋白的功能和用途,并为深入研究链霉亲和素蛋白的进化提供参考。The Streptavidin/Biotin system , which has the highest affinity to date, has been widely used in biological research. In this paper, the evolution of two related systems, Streptavidin/Biotin and Strep- Tactin/Strep-tag, were summarized in detail from the structural modification of the streptavidin protein and the optimization of affinity peptide tags. By comparing the advantages and disadvantages of various mutants of the streptavidin protein , we aim to provide a valuable reference to select a suitable streptavidin mutant in practical applications. Herein we review the evolution of the streptavidin protein, facilitating a more accurate understanding of the function and use of various streptavidin proteins in the market, and provide a reference for further research on the evolution of streptavidin.

关 键 词：链霉亲和素 亲和系统 亲和肽标签 蛋白质进化 

分 类 号：Q51[生物学—生物化学] Q71