Protective effect and mechanisms of Weining granule on N-methyl-N’-nitro-N-nitrosoguanidine-induced gastric cancer in rats  

作　　者：Deng Xin Liang Xingqiu Zhou Xiaoxiao Jiang Manjun Liang Mingkun Wang Xinyuan Zhao Xiaofang Fu Lei Liang Jian 

机构地区：[1]Key Laboratory of basic research in Guangxi Traditional Chinese Medicine, Nanning 541100, China [2]School of Basic Medical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, China [3]the Department of Science and Technology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 541100, China [4]Integrative Medicine Institute, Hunan University of Chinese Medicine, Changsha 410208, China [5]Medical college, Guangxi university, Nanning 530004, China

出　　处：《Journal of Traditional Chinese Medicine》2019年第3期393-401,共9页中医杂志（英文版）

基　　金：Supported by the National Nature Science Foundation of China(No.81660743);the Project of Guangxi Scholars;the Special Project of Guangxi Traditional Chinese Medicine Administration Bureau(No.GZYZ-10-18);the Project of Guangxi Young Teacher Fundamental Ability Promotion(No.2017KY0298)

摘　　要：OBJECTIVE:To investigate the protective effect and molecular mechanisms of Weining granule on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric cancer in rats.METHODS:A total of sixty healthy male wistar rats were randomly divided into five groups,including control group (CG),gastric cancer model group (MG),low-dose Weining granule treated group (LWT),medium-dose Weining granule treated group (MWT),and high-dose Weining granule treated group (HWT).Except the control group,the other groups were treated with MNNG to establish a rat model of gastric cancer.Low-dose Weining granule treated group,medium-dose Weining granule treated group,and high-dose Weining granule treated group were fed 9.0,18.0 and 36.0 g/kg Weining granule,respectively.Histopathologic and molecular biologic technology were adopted to determine the protective effect of Weining granule on MNNG-induced gastric cancer in rats.The pathological changes of gastrointestinal tissue were observed.Meanwhile,the differential expression of proliferation,apoptosis and angiogenesis markers were determined,including proliferating cell nuclear antigen (PCNA),pokemon,cyclin D1,B-cell lymphoma-2 (Bcl-2),caspase-3,phosphatase and tensin homolog (PTEN) and vascular endothelial growth factor (VEGF).RESULTS.:After the MNNG treated,the pathological changes of stomach tissue were improved noticeably,including the intestinal metaplasia and atypic hyperplasia.The experiment was completed in.58 rats (96.67%).As compared with gastric cancer model group,the general states of rats were improved significantly after treated with different dose Weining granule.Moreover,treatment with different doses of Weining granule could inhibit the protein and mRNA expression of PCNA,pokemon,cyclin D1,Bcl-2,and VEGF,while increase caspase-3 and PTEN (P < 0.01).CONCLUSION:Weining granule could improve gastric cancer by suppressing cell proliferation,promoting tumor cell apoptosis,and inhibiting angiogenesis.OBJECTIVE:To investigate the protective effect and molecular mechanisms of Weining granule on N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)-induced gastric cancer in rats.METHODS:A total of sixty healthy male wistar rats were randomly divided into five groups,including control group(CG),gastric cancer model group(MG),low-dose Weining granule treated group(LWT),medium-dose Weining granule treated group(MWT),and high-dose Weining granule treated group(HWT).Except the control group,the other groups were treated with MNNG to establish a rat model of gastric cancer.Low-dose Weining granule treated group,medium-dose Weining granule treated group,and high-dose Weining granule treated group were fed 9.0,18.0 and 36.0 g/kg Weining granule,respectively.Histopathologic and molecular biologic technology were adopted to determine the protective effect of Weining granule on MNNG-induced gastric cancer in rats.The pathological changes of gastrointestinal tissue were observed.Meanwhile,the differential expression of proliferation,apoptosis and angiogenesis markers were determined,including proliferating cell nuclear antigen(PCNA),pokemon,cyclin D1,B-cell lymphoma-2(Bcl-2),caspase-3,phosphatase and tensin homolog(PTEN)and vascular endothelial growth factor(VEGF).RESULTS:After the MNNG treated,the pathological changes of stomach tissue were improved noticeably,including the intestinal metaplasia and atypic hyperplasia.The experiment was completed in 58 rats(96.67%).As compared with gastric cancer model group,the general states of rats were improved significantly after treated with different dose Weining granule.Moreover,treatment with different doses of Weining granule could inhibit the protein and mRNA expression of PCNA,pokemon,cyclin D1,Bcl-2,and VEGF,while increase caspase-3 and PTEN(P<0.01).CONCLUSION:Weining granule could improve gas tric cancer by suppressing cell proliferation,promoting tumor cell apoptosis,and inhibiting angiogenesis.

关 键 词：STOMACH neoplasms METHYLNITRONITROSOGUANIDINE Cell proliferation Apoptosis Angiogenesis inhibitors Weining GRANULE 

分 类 号：R[医药卫生]