转录因子叉头框C2基因C-512T多态性与非酒精性脂肪性肝病患者相关代谢因素的分析  

Analysis between FOXC2 gene C-512T polymorphism and metabolic factors in patients with non-alcoholic fatty liver disease

作　　者：陈冰[1] 陈婷[1] 黄红艳[2] 周碧燕[3] 何文军[4] 赵丽萍[3] 杜永光 潘甘桂 CHEN Bing;CHEN Ting;HUANG Hongyan(Department of Endocrinology,First People's Hospital of Nanning City,Nanning 530021,China)

机构地区：[1]南宁市第一人民医院内分泌科,530021 [2]南宁市第一人民医院体检科,530021 [3]南宁市第一人民医院检验科,530021 [4]南宁市第一人民医院放免检验科,530021

出　　处：《中国糖尿病杂志》2019年第7期486-490,共5页Chinese Journal of Diabetes

基　　金：南宁市科学研究与技术开发计划项目(200501078c)

摘　　要：目的探讨转录因子叉头框C2(FOXC2)基因C-512T多态性与非酒精性脂肪性肝病(NAFLD)及其相关代谢因素的关系。方法应用聚合酶连反应-限制性片段长度多态性(PCRRFLP)方法,对269名正常对照组(NC)和237例NAFLD患者(NAFLD组)的FOXC2基因C-512T多态性进行测定,同时进行体表测量、生化指标及腹部B超检查。结果与NC组比较,NAFLD组FOXC2基因C-512T多态性的基因型频率分布差异无统计学意义(χ^2=4.455,P=0.108);而NAFLD组T等位基因频率降低(χ^2=3.638,P=0.048);NAFLD组CC型患者TG、HOMA-IR高于TT型患者,差异有统计学意义(P<0.05)。Logistic回归分析显示,CC基因型和HOMA-IR是NAFLD的影响因素。结论FOXC2基因C-512T多态性CC基因型可能与NAFLD发生有关。Objective To investigate the relationship between FOXC2 gene C-512 T polymorphism and metabolic factors in patients with non-alcoholic fatty liver disease(NAFLD).Methods A total of506 subjects were enrolled in this study and divided into two groups:normal control(NC)group(n=269)and NAFLD group(n=237). FOXC2 gene C-512 T polymorphism was tested by restriction fragment length polymorphism(PCR-RFLP)in all the subjects. Meanwhile,physical examination,biochemical tests and abdominal ultrasound examination were tested.Results There was no significant difference between genotypes frequency distribution of the FOXC2 gene C-512 T polymorphism in the NAFLD group and NC group(χ^2=4. 455,P=0. 108). The T allele frequency was lower in the group of NAFLD than in NC group(χ^2= 3. 638,P=0. 048). TG and HOMA-IR were higher in CC than in TT genotype in NAFLD group(P<0. 05). Logistic analysis showed that CC genotype and HOMA-IR were the risk factors for NAFLD.Conclusion CC genotype of FOXC2 gene C-512 T polymorphism may be involved in the pathogenesis of NAFLD.

关 键 词：转录因子叉头框C基因 多态性 非酒精性脂肪性肝病 胰岛素抵抗 

分 类 号：R575.5[医药卫生—消化系统]