DNA倍体分析对胰腺占位性病变的诊断价值  

Diagnostic Values of DNA Ploidy Detection in Pancreatic Space-occupying Lesions

作　　者：周志姣[1] 洪敏 田力[2] 邹琼[1] ZHOU Zhi-jiao;HONG Ming;Tiart Li(Department of Pathology y Third Xiangya Hospitals Central South University,Changsha, 410005,China)

机构地区：[1]中南大学湘雅三医院病理科,湖南长沙410005 [2]中南大学湘雅三医院消化内科,湖南长沙410005

出　　处：《医学临床研究》2019年第7期1267-1269,共3页Journal of Clinical Research

基　　金：湖南省长沙市科技计划项目(kq1701084).

摘　　要：【目的】探讨DNA倍体分析在胰腺占位性病变中的诊断价值。【方法】收集本院肢腺占位性病变 148例,超声内镋引导下细针穿刺获取病变组织,DNA倍体自动检测分析仪对染色切片中的每个细胞核进行. DNA倍体分析,并与细胞学和组织学结果对照分析。【结果】148例病变经穿剌均得到理想的标本并获得细胞学诊断,64例胰腺非肿瘤性病变无DNA倍体异常细跑,84例肢腺肿瘤性病交中发现79例DNA倍体异常细胞,阳性率94.0%(79/84),明显高于非肿瘤性病变的0.0%(0/64),胰腺肿痼性病变的DNA倍体阳性率为 94%(79/84),显著高于细胞学诊断的76.2%(64/84),其差异有统计学意义(P <0.05),【结论】DNA倍体分析可提高胰腺占位性_变检測的阳性率,对胰腺占位性病变患者的诊断提供参考价值。【Objective】To explore the diagnostic values of DNA ploidy analysis in pancreatic space-occup- ying lesions.【Methods】A total of 148 patients with pancreatic space-occupying lesions from the Third Xian- gya Hospital, Central South University were collected. EUS-FNA (ultrasound endoscopically guided final nee-dle aspiration) were performed in all cases. DNA ploidy analysis system was used to analyze DNA content in nucleus. iResultsJEUS-FNA was successfully" performed and adequate specimen for cytological diagnosis were obtained in 148 patients. Results showed that 64 patients with non-neoplastic lesion of the pancreas had no DNA ploidy abnormal cells and out of the 84 patients with pancreatic neoplastic lesions, 79 cases were found with DNA ploidy abnormal cells.The aneuploidy positive rate was (94.0%(79/84))in 84 pancreatic neoplastic lesion, which was significantly higher than non-neoplastic lesion of pancreas 0.0%(0/64). The difference was statistically significant between the two groups( P <0.05).【Conclusion】DNA ploidy analysis can improve the positive detection rate of pancreatic space-occupying lesions. It has clinical guidance for the diagnosis of pa-tients with pancreatic space-occupying lesions.

关 键 词：胰腺疾病/诊断 DNA/分析 

分 类 号：R576[医药卫生—消化系统]