华卟啉钠光动力疗法对人舌鳞癌SCC-15细胞的作用  

The effects of DVDMS-PDT on tongue squamous cell carcinoma SCC-15 cells

作　　者：马垚 张磊[1] 黄宇思 刘鑫 MA Yao;ZHANG Lei;HUANG Yusi;LIU Xin(College of Stomatology,Harbin Medical University,150001,China)

机构地区：[1]哈尔滨医科大学口腔医学院

出　　处：《实用口腔医学杂志》2019年第4期562-567,共6页Journal of Practical Stomatology

基　　金：哈尔滨医科大学附属第一医院科研基金(编号:2014B19)

摘　　要：目的:研究华卟啉钠( DVDMS)介导的光动力治疗( DVDMS-PDT)对舌鳞癌细胞SCC-15 体外增殖、凋亡及迁移能力的影响。方法:体外培养SCC-15 细胞,CCK-8 法检测DVDMS 药物毒性,酶标仪M3 及荧光显微镜确定孵育时间。将SCC-15细胞随机分成空白对照组、单药组、单光组、DVDMS-PDT 组,CCK-8 检测细胞存活率,流式检测细胞凋亡,划痕实验检测细胞的迁移能力。结果: DVDMS 浓度≤2 μg /ml 对SCC-15 细胞无明显杀伤作用,其在SCC-15 细胞内的最佳孵育时间为6 h,浓度为2 μg /ml 的DVDMS-PDT 对SCC-15 呈剂量依赖性抑制作用( P < 0. 05)。DVDMS-PDT 诱导SCC-15 凋亡率显著高于对照组、单药组和单光组( P < 0. 001)而划痕愈合百分比明显低于各对照组( P < 0. 05)。结论: DVDMS-PDT 能抑制舌鳞癌SCC-15细胞的增殖和迁移能力,促进细胞凋亡。Objective: To study the effects of Sinoporphyrin Sodium-Photodynamic therapy( DVDMS-PDT) on the proliferation,apoptosis and migration of tongue squamous cell carcinoma SCC-15 cells in vitro. Methods: SCC-15 cells were routinely cultured in vitro. CCK-8 was used to examine the cytotoxicity of DVDMS. Elisa reader M3 and fluorescence microscope were used to determine the incubation time of DVDMS with the cells. The cells were divided into 4 groups: blank control group,DVDMS group,light group and DVDMS-PDT group. CCK-8 was used to examine the cell viability. Cell migration was detected by wound healing assay while cell apoptosis was detected by flow cytometry. Results: DVDMS at the concentration ≤2 μg /ml showed no inhibition effect on SCC-15 cells and the maximal uptake of DVDMS occurred within 6 h of culture. DVDMS-PDT at 2 μg /ml inhibited the proliferation of SCC-15 cells in a dose-dependent manner( P < 0. 05). The apoptotic rate in the DVDMS-PDT group was significantly higher than that in the other groups( P < 0. 001) while the wound healing percentage was decreased( P < 0. 05). Conclusion: DVDMS-PDT can inhibit the proliferation and migration,but promote the apoptosis of SCC-15 cells.

关 键 词：舌鳞癌 华卟啉钠(DVDMS) 光动力疗法 细胞凋亡 细胞迁移 

分 类 号：R739.8[医药卫生—肿瘤]