基于信息熵理论的正交设计优化消乳增胶囊的提取工艺  

Optimization of extraction process of Xiaoruzeng Capsules based on orthogonal design in information entropy theory

作　　者：陈丽津 汤浩[1] 石磊[1] 钱倩 蒲君峰[1] CHEN Li-jin;TANG Hao;SHI Lei;QIAN Qian;PU Jun-feng(Department of Pharmacy, Gansu Provincial Hospital, Lanzhou 730000, China;Faculty of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China)

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000 [2]甘肃中医药大学药学院,甘肃兰州730000

出　　处：《中草药》2019年第14期3345-3350,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的优选消乳增胶囊中7味药材的提取工艺参数。方法采用HPLC测定芍药苷、阿魏酸、橙皮苷、欧前胡素、藁本内酯的含量,以此5种指标成分的提取率、药材提取的干膏得率和提取物指纹图谱相似度为综合评价指标,通过正交试验考察乙醇浓度、提取溶剂倍量、提取时间和提取次数对提取工艺的影响,采用信息熵赋权法确定各指标的客观权重,优选消乳增胶囊中7味药材的提取工艺参数。结果根据综合评分结果,确定该制剂最佳提取工艺为选用6倍量的50%乙醇,煎煮2次,每次2 h。3批验证综合评分均值为99.72,RSD为0.24%。结论优选后的工艺提取率高、稳定性和重复性好,适用于该制剂的大批量生产。Objective To optimize the extraction process parameters of seven herbs in Xiaoruzeng Capsules (XC). Methods The content of paeoniflorin, ferulic acid, hesperidin, imperatorin and ligustilide was determined by HPLC. The extraction rate of five kinds of index components, the dry extract yield of extracted herbs and the fingerprint similarity of extracts were comprehensively evaluated. The orthogonal experiment was carried out to investigate the effects of ethanol concentration, extraction solvent, extraction time and extraction times on the extraction process. The information entropy weighting method was used to determine the objective weight of each index, and the extraction process parameters of seven herbs in XC were optimized. Results According to the comprehensive scoring results, it was determined that the best extraction process of the preparation was to add six times the amount of 50% ethanol, and decocted two times for 2 h each time. The average score of the three batches of verification scores was 99.72, and the RSD was 0.24%. Conclusion The preferred process has high extraction rate, with good stability and repeatability, which is suitable for mass production of the preparation.

关 键 词：消乳增胶囊 指纹图谱 正交试验 信息熵 提取工艺 芍药苷 阿魏酸 橙皮苷 欧前胡素 藁本内酯 

分 类 号：R284.2[医药卫生—中药学]